cDNA cloning and gene expression of ascorbate oxidase in tobacco
Copyright policy )cDNA cloning and gene expression of ascorbate oxidase in tobacco
A cDNA clone for ascorbate oxidase (AAO) has been isolated from a cDNA library of tobacco (Nicotiana tabacum) cells. The identity of the amino acid sequence deduced from tobacco AAO cDNA to that from pumpkin AAO cDNA was 68%, which was much lower than the identity (80%) between pumpkin and cucumber AAO. AAO activity in tobacco cells was much lower than that in pumpkin cells, whereas the immunoreactive protein in tobacco cells was more abundant than that in pumpkin cells. We suppose that AAO protein in tobacco cells may be less active than that in pumpkin cells. Genomic Southern blotting suggested that AAO in tobacco was encoded by a single-copy gene. Nothern blotting revealed that mRNA of AAO was highly expressed in young and growing tissues of tobacco plant.
- Hiroshima University Japan
Nicotiana, DNA, Complementary, Sequence Homology, Amino Acid, Molecular Sequence Data, Gene Expression, Sequence Analysis, DNA, Blotting, Southern, Plants, Toxic, Species Specificity, Ascorbate Oxidase, Amino Acid Sequence, Cloning, Molecular, Cells, Cultured, Genome, Plant, Plant Proteins
Nicotiana, DNA, Complementary, Sequence Homology, Amino Acid, Molecular Sequence Data, Gene Expression, Sequence Analysis, DNA, Blotting, Southern, Plants, Toxic, Species Specificity, Ascorbate Oxidase, Amino Acid Sequence, Cloning, Molecular, Cells, Cultured, Genome, Plant, Plant Proteins
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).42 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!