cDNA cloning and expression of rsca1, the rat counterpart of the human spinocerebellar ataxia type 1 gene
Copyright policy )cDNA cloning and expression of rsca1, the rat counterpart of the human spinocerebellar ataxia type 1 gene
Spinocerebellar ataxia type 1 (SCA1) is a neurodegenerative disorder caused by an expanded and unstable (CAG) > 40 repeat within a gene of unknown function. We isolated the complete coding region of the rat SCA1 gene (rsca1), the 5'-untranslated region (UTR) and 1.3 kb of the 3'-UTR. The rat sequence exhibits 90% peptide identity to the human counterpart. In comparison to human, the rat (CAG)n block is reduced to two trinucleotide motifs preceded by three different proline codons not present in man. Furthermore, we investigated the expression of rsca1 in different rat tissues. The rsca1 gene is predominantly expressed in brain throughout all developmental stages. In situ hybridizations reveal high levels of expression in various regions of the adult rat brain, including cerebellum, hippocampus and cortex.
- Ruhr University Bochum Germany
Brain Chemistry, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Brain, Nuclear Proteins, Nerve Tissue Proteins, Rats, Inbred Strains, Blotting, Northern, Rats, Evolution, Molecular, Ataxins, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Ataxin-1, Conserved Sequence, In Situ Hybridization
Brain Chemistry, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Brain, Nuclear Proteins, Nerve Tissue Proteins, Rats, Inbred Strains, Blotting, Northern, Rats, Evolution, Molecular, Ataxins, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Ataxin-1, Conserved Sequence, In Situ Hybridization
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).9 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!