The influence of the acyl chain composition of cardiolipin on the stability of mitochondrial complexes; An unexpected effect of cardiolipin in α-ketoglutarate dehydrogenase and prohibitin complexes
Copyright policy )The influence of the acyl chain composition of cardiolipin on the stability of mitochondrial complexes; An unexpected effect of cardiolipin in α-ketoglutarate dehydrogenase and prohibitin complexes
The role of cardiolipin acyl chain composition in assembly/stabilization of mitochondrial complexes was investigated using three yeast deletion mutants (acb1Delta strain; taz1Delta strain; and acb1Deltataz1Delta strain). Deletion of the TAZ1 gene, involved in cardiolipin acyl chain remodeling, is known to increase the content of monolyso-cardiolipin (MLCL) at the expense of CL, and to decrease the unsaturation of the remaining CL. Deletion of the ACB1 gene encoding the acyl-CoA-binding protein, involved in fatty acid elongation, decreases the average length of the CL acyl chains. Furthermore, a TAZ1ACB1 double deletion mutant strain was used in this study which has both a decrease in the length of the CL acyl chains and an increase in MLCL. BN/SDS PAGE analysis revealed that cardiolipin is important for the prohibitin-m-AAA protease complex, the alpha-ketoglutarate dehydrogenase complex and respiratory chain supercomplexes. The results indicate that the decreased level of complexes in taz1Delta and acb1Deltataz1Delta mitochondria is due to a decreased content of CL or the presence of MLCL.
- Utrecht University Netherlands
Yeast mitochondria, Supercomplex, Cardiolipins, Acylation, Blue native PAGE, Saccharomyces cerevisiae, Acyl-CoA binding protein, Mass Spectrometry, Electron Transport, Mitochondrial Proteins, Prohibitins, Ketoglutarate Dehydrogenase Complex, Taffazin, Diazepam Binding Inhibitor, Metalloendopeptidases, Mitochondria, Repressor Proteins, Multiprotein Complexes, Cardiolipin, Mutation, Electrophoresis, Polyacrylamide Gel, Gene Deletion, Chromatography, Liquid
Yeast mitochondria, Supercomplex, Cardiolipins, Acylation, Blue native PAGE, Saccharomyces cerevisiae, Acyl-CoA binding protein, Mass Spectrometry, Electron Transport, Mitochondrial Proteins, Prohibitins, Ketoglutarate Dehydrogenase Complex, Taffazin, Diazepam Binding Inhibitor, Metalloendopeptidases, Mitochondria, Repressor Proteins, Multiprotein Complexes, Cardiolipin, Mutation, Electrophoresis, Polyacrylamide Gel, Gene Deletion, Chromatography, Liquid
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