MCF-7 breast carcinoma cells express ryanodine receptor type 1: functional characterization and subcellular localization
Copyright policy )MCF-7 breast carcinoma cells express ryanodine receptor type 1: functional characterization and subcellular localization
Breast carcinoma-derived MCF-7 cells are frequently used in biomedical research. However, few reports exist regarding the characterization of signaling mechanisms in these cancerous cells involved in intracellular Ca(2+) dynamics. Consequently, the aim of these experiments was to characterize the ryanodine receptor/Ca(2+) release channel (RyR) present in MCF-7 cells. Ryanodine (100 nM), cADPR (5 microM), and caffeine (10 mM) promoted cytoplasmic Ca(2+) mobilization; in contrast, ryanodine at inhibitory concentration (100 microM) decreased the basal Ca(2+) level. Fluorescent probes demonstrated that RyR is located mainly in endomembranes. Some degree of co-localization with inositol trisphosphate receptor (IP(3)R) was observed, whereas coincidence with thapsigargin-sensitive Ca(2+)-ATPase (SERCA) was more limited. Molecular cloning resulted in the detection exclusively of RyR isoform 1. For the first time, it is shown that MCF-7 cells express functional RyR.
Base Sequence, Molecular Sequence Data, Breast Neoplasms, Ryanodine Receptor Calcium Release Channel, Mice, Cell Line, Tumor, Animals, Humans, Inositol 1,4,5-Trisphosphate Receptors, Protein Isoforms, Thapsigargin, Calcium, Female, Enzyme Inhibitors, Sequence Alignment, Aged, Signal Transduction, Subcellular Fractions
Base Sequence, Molecular Sequence Data, Breast Neoplasms, Ryanodine Receptor Calcium Release Channel, Mice, Cell Line, Tumor, Animals, Humans, Inositol 1,4,5-Trisphosphate Receptors, Protein Isoforms, Thapsigargin, Calcium, Female, Enzyme Inhibitors, Sequence Alignment, Aged, Signal Transduction, Subcellular Fractions
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