In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p
Copyright policy )In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p
Isw1p and Isw2p are budding yeast homologs of the DrosophilaISWI chromatin-remodeling ATPase. Using indirect-end-label and chromatin immunoprecipitation analysis, we show both independent and cooperative Isw1p- and Isw2p-mediated positioning of short nucleosome arrays in gene-regulatory elements at a variety of transcription units in vivo. We present evidence that both yeast ISWI complexes regulate developmental responses to starvation and that for Isw2p, recruitment by different DNA-binding proteins controls meiosis and haploid invasive growth.
- University of Oxford United Kingdom
Adenosine Triphosphatases, DNA Footprinting, Nucleic Acid Hybridization, Saccharomyces cerevisiae, Blotting, Northern, Chromatin, Fungal Proteins, Meiosis, Micrococcal Nuclease, DNA, Fungal, Transcription Factors
Adenosine Triphosphatases, DNA Footprinting, Nucleic Acid Hybridization, Saccharomyces cerevisiae, Blotting, Northern, Chromatin, Fungal Proteins, Meiosis, Micrococcal Nuclease, DNA, Fungal, Transcription Factors
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