Mouse thromboxane A2 receptor: cDNA cloning, expression and Northern blot analysis
Copyright policy )Mouse thromboxane A2 receptor: cDNA cloning, expression and Northern blot analysis
A cDNA clone for the mouse thromboxane A2 receptor was isolated from mouse lung cDNA library. The cDNA has a 1,023 base pair open reading frame which encodes a protein of 341 amino acid residues. STA2 and U-46619 induced inward current in Xenopus laevis oocytes injected with the transcript of the clone. Specific binding of [3H]S-145 was found in membranes of COS-1 cells transfected with the cDNA (Kd = 3.3 nM) and was displaced with unlabeled prostaglandins and thromboxane analogues in the order of S-145 greater than STA2 greater than U-46619 greater than PGD2 greater than PGF2 alpha = PGE2. Northern blot analysis demonstrated that thromboxane A2 receptor mRNA is expressed abundantly in thymus, spleen and lung.
- Kyoto University Japan
Male, Base Sequence, Cell Membrane, Molecular Sequence Data, DNA, Blotting, Northern, Binding, Competitive, Cell Line, Kinetics, Mice, Oligodeoxyribonucleotides, Organ Specificity, Oocytes, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Poly A, Lung, Gene Library
Male, Base Sequence, Cell Membrane, Molecular Sequence Data, DNA, Blotting, Northern, Binding, Competitive, Cell Line, Kinetics, Mice, Oligodeoxyribonucleotides, Organ Specificity, Oocytes, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Poly A, Lung, Gene Library
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).181 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!