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Publication . Article . 2010

The role of Vδ2-negative γδ T cells during cytomegalovirus reactivation in recipients of allogeneic stem cell transplantation

Knight, Andrea; Madrigal, Alejandro J.; Grace, Sarah; Sivakumaran, Janani; Kottaridis, Panagiotis; Mackinnon, Stephen; Travers, Paul J.; +1 Authors
Published: 23 Sep 2010
Publisher: American Society of Hematology
Abstract
Abstract Reactivation of cytomegalovirus (CMV) remains a serious complication after allogeneic stem cell transplantation, but the role of γδ T cells is undefined. We have studied the immune reconstitution of Vδ2negative (Vδ2neg) γδ T cells, including Vδ1 and Vδ3 subsets and Vδ2positive (Vδ2pos) γδ T cells in 40 patients during the first 24 months after stem cell transplantation. Significant long-term expansions of Vδ2neg but not Vδ2pos γδ T cells were observed during CMV reactivation early after transplantation, suggesting direct involvement of γδ T cells in anti-CMV immune responses. Similarly, significantly higher numbers of Vδ2neg γδ T cells were detected in CMV-seropositive healthy persons compared with seronegative donors; the absolute numbers of Vδ2pos cells were not significantly different. The expansion of Vδ2neg γδ T cells appeared to be CMV-related because it was absent in CMV-negative/Epstein-Barr virus-positive patients. T-cell receptor-δ chain determining region 3 spectratyping of Vδ2neg γδ T cells in healthy subjects and patients showed restricted clonality. Polyclonal Vδ2neg cell lines generated from CMV-seropositive healthy donors and from a recipient of a graft from a CMV-positive donor lysed CMV-infected targets in all cases. Our study shows new evidence for role of γδ T cells in the immune response to CMV reactivation in transplantation recipients.
Subjects by Vocabulary

Microsoft Academic Graph classification: T cell medicine.anatomical_structure medicine Stem cell Immunology Transplantation Immune system Hematopoietic stem cell transplantation medicine.medical_treatment Bone marrow Virology Biology Antigen Herpesviridae medicine.disease_cause

Medical Subject Headings: virus diseases

Subjects

Cell Biology, Hematology, Immunology, Biochemistry

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Article . 2010