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Publication . Article . 2020

Role of CaMKII and sarcolipin in muscle adaptations to strength training with different levels of fatigue in the set

Miriam Martinez-Canton; Angel Gallego-Selles; Miriam Gelabert-Rebato; Marcos Martin-Rincon; Fernando Pareja-Blanco; David Rodríguez-Rosell; David Morales-Alamo; +4 Authors
Closed Access
Published: 02 Oct 2020 Journal: Scandinavian Journal of Medicine & Science in Sports, volume 31, pages 91-103 (issn: 0905-7188, eissn: 1600-0838, Copyright policy )
Publisher: Wiley
Abstract

Strength training promotes a IIX-to-IIA shift in myosin heavy chain (MHC) composition, likely due to changes in sarcoplasmic [Ca2+ ] which are sensed by CaMKII. Sarcoplasmic [Ca2+ ] is in part regulated by sarcolipin (SLN), a small protein that when overexpressed in rodents stimulates mitochondrial biogenesis and a fast-to-slow fiber type shift. The purpose of this study was to determine whether CaMKII and SLN are involved in muscle phenotype and performance changes elicited by strength training. Twenty-two men followed an 8-week velocity-based resistance training program using the full squat exercise while monitoring repetition velocity. Subjects were randomly assigned to two resistance training programs differing in the repetition velocity loss allowed in each set: 20% (VL20) vs 40% (VL40). Strength training caused muscle hypertrophy, improved 1RM and increased total CaMKII protein expression, particularly of the δD isoform. Phospho-Thr287 -CaMKII δD expression increased only in VL40 (+89%), which experienced greater muscle hypertrophy, and a reduction in MHC-IIX percentage. SLN expression was increased in VL20 (+33%) remaining unaltered in VL40. The changes in phospho-Thr287 -CaMKII δD were positively associated with muscle hypertrophy and the number of repetitions during training, and negatively with the changes in MHC-IIX and SLN. Most OXPHOS proteins remained unchanged, except for NDUFB8 (Complex I), which was reduced after training (-22%) in both groups. The amount of fatigue allowed in each set critically influences muscle CaMKII and SLN responses and determines muscle phenotype changes. With lower intra-set fatigue, the IIX-to-IIA MHC shift is attenuated.

Subjects by Vocabulary

Microsoft Academic Graph classification: Ca2+/calmodulin-dependent protein kinase Chemistry Endocrinology medicine.medical_specialty medicine Internal medicine Sarcoplasm Myosin Muscle hypertrophy Mitochondrial biogenesis Strength training Skeletal muscle medicine.anatomical_structure Sarcolipin

Subjects

Physical Therapy, Sports Therapy and Rehabilitation, Orthopedics and Sports Medicine

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