publication . Article . Other literature type . 2015

A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae

Hammond, Andrew; Galizi, Roberto; Kyrou, Kyros; Simoni, Alekos; Siniscalchi, Carla; Katsanos, Dimitris; Gribble, Matthew; Baker, Dean; Marois, Eric; Russell, Steve; ...
  • Published: 07 Dec 2015
  • Country: United Kingdom
Abstract
Gene-drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years [AU please provide a real estimate, this seems vague]. We describe CRISPR-Cas9 endonuclease constructs that function as gene-drive systems in Anopheles gambiae, the main vector for malaria [AU:OK?]. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene-drive constructs designed to target and edit each gene [AU:OK?]. For each locus targeted we observed strong gene drive at the molecular l...
Subjects
free text keywords: Animals, Anopheles gambiae, Malaria, Insect Vectors, Female, Clustered Regularly Interspaced Short Palindromic Repeats, Article, Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, HOMING ENDONUCLEASE GENES, DROSOPHILA, SPECIFICITY, EXPRESSION, NUCLEASES, GERMLINE, TOOLS, Anopheles, MD Multidisciplinary, Q1, QH, Biotechnology, Molecular Medicine, Applied Microbiology and Biotechnology, Bioengineering, Biomedical Engineering, Gene drive, biology.organism_classification, biology, Parasite hosting, Genetics, Vector (epidemiology), CRISPR, Cas9, medicine.disease, medicine, Gene Drive Technology
Funded by
EC| VECSYN
Project
VECSYN
Synthetic species of the mosquito vectors of human disease: from hybrid genetics to a new type of vector control
  • Funder: European Commission (EC)
  • Project Code: 335724
  • Funding stream: FP7 | SP2 | ERC
31 references, page 1 of 3

Burt, A. Site-specific selfish genes as tools for the control and genetic engineering of natural populations. Proc Biol Sci. 2003; 270: 921-928 [OpenAIRE] [PubMed]

Windbichler, N. A synthetic homing endonuclease-based gene drive system in the human malaria mosquito. Nature. 2011; 473: 212-215 [OpenAIRE] [PubMed]

Simoni, A. Development of synthetic selfish elements based on modular nucleases in Drosophila melanogaster. Nucleic Acids Res. 2014; 42: 7461-7472 [OpenAIRE] [PubMed]

Fu, Y, Sander, JD, Reyon, D, Cascio, VM, Joung, JK. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol. 2014; 32: 279-284 [OpenAIRE] [PubMed]

Hsu, PD. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol. 2013; 31: 827-832 [OpenAIRE] [PubMed]

Jinek, M. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012; 337: 816-821 [OpenAIRE] [PubMed]

Esvelt, KM, Smidler, AL, Catteruccia, F, Church, GM. Concerning RNA-guided gene drives for the alteration of wild populations. Elife. 2014: e03401 [OpenAIRE] [PubMed]

DiCarlo, JE, Chavez, A, Dietz, SL, Esvelt, KM, Church, GM. Safeguarding CRISPR-Cas9 gene drives in yeast. Nat Biotechnol. 2015 [OpenAIRE]

Gantz, VM, Bier, E. Genome editing. The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations. Science. 2015; 348: 442-444 [OpenAIRE] [PubMed]

Deredec, A, Burt, A, Godfray, HC. The population genetics of using homing endonuclease genes in vector and pest management. Genetics. 2008; 179: 2013-2026 [OpenAIRE] [PubMed]

Baker, DA. A comprehensive gene expression atlas of sex- and tissue-specificity in the malaria vector, Anopheles gambiae. BMC Genomics. 2011; 12: 296 [OpenAIRE] [PubMed]

Giraldo-Calderon, GI. VectorBase: an updated bioinformatics resource for invertebrate vectors and other organisms related with human diseases. Nucleic Acids Res. 2015; 43: D707-713 [OpenAIRE] [PubMed]

Claycomb, JM, Benasutti, M, Bosco, G, Fenger, DD, Orr-Weaver, TL. Gene amplification as a developmental strategy: isolation of two developmental amplicons in Drosophila. Dev Cell. 2004; 6: 145-155 [OpenAIRE] [PubMed]

Hong, CC, Hashimoto, C. The maternal nudel protein of Drosophila has two distinct roles important for embryogenesis. Genetics. 1996; 143: 1653-1661 [OpenAIRE] [PubMed]

Bateman, JR, Lee, AM, Wu, CT. Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Genetics. 2006; 173: 769-777 [OpenAIRE] [PubMed]

31 references, page 1 of 3
Abstract
Gene-drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years [AU please provide a real estimate, this seems vague]. We describe CRISPR-Cas9 endonuclease constructs that function as gene-drive systems in Anopheles gambiae, the main vector for malaria [AU:OK?]. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene-drive constructs designed to target and edit each gene [AU:OK?]. For each locus targeted we observed strong gene drive at the molecular l...
Subjects
free text keywords: Animals, Anopheles gambiae, Malaria, Insect Vectors, Female, Clustered Regularly Interspaced Short Palindromic Repeats, Article, Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, HOMING ENDONUCLEASE GENES, DROSOPHILA, SPECIFICITY, EXPRESSION, NUCLEASES, GERMLINE, TOOLS, Anopheles, MD Multidisciplinary, Q1, QH, Biotechnology, Molecular Medicine, Applied Microbiology and Biotechnology, Bioengineering, Biomedical Engineering, Gene drive, biology.organism_classification, biology, Parasite hosting, Genetics, Vector (epidemiology), CRISPR, Cas9, medicine.disease, medicine, Gene Drive Technology
Funded by
EC| VECSYN
Project
VECSYN
Synthetic species of the mosquito vectors of human disease: from hybrid genetics to a new type of vector control
  • Funder: European Commission (EC)
  • Project Code: 335724
  • Funding stream: FP7 | SP2 | ERC
31 references, page 1 of 3

Burt, A. Site-specific selfish genes as tools for the control and genetic engineering of natural populations. Proc Biol Sci. 2003; 270: 921-928 [OpenAIRE] [PubMed]

Windbichler, N. A synthetic homing endonuclease-based gene drive system in the human malaria mosquito. Nature. 2011; 473: 212-215 [OpenAIRE] [PubMed]

Simoni, A. Development of synthetic selfish elements based on modular nucleases in Drosophila melanogaster. Nucleic Acids Res. 2014; 42: 7461-7472 [OpenAIRE] [PubMed]

Fu, Y, Sander, JD, Reyon, D, Cascio, VM, Joung, JK. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol. 2014; 32: 279-284 [OpenAIRE] [PubMed]

Hsu, PD. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol. 2013; 31: 827-832 [OpenAIRE] [PubMed]

Jinek, M. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012; 337: 816-821 [OpenAIRE] [PubMed]

Esvelt, KM, Smidler, AL, Catteruccia, F, Church, GM. Concerning RNA-guided gene drives for the alteration of wild populations. Elife. 2014: e03401 [OpenAIRE] [PubMed]

DiCarlo, JE, Chavez, A, Dietz, SL, Esvelt, KM, Church, GM. Safeguarding CRISPR-Cas9 gene drives in yeast. Nat Biotechnol. 2015 [OpenAIRE]

Gantz, VM, Bier, E. Genome editing. The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations. Science. 2015; 348: 442-444 [OpenAIRE] [PubMed]

Deredec, A, Burt, A, Godfray, HC. The population genetics of using homing endonuclease genes in vector and pest management. Genetics. 2008; 179: 2013-2026 [OpenAIRE] [PubMed]

Baker, DA. A comprehensive gene expression atlas of sex- and tissue-specificity in the malaria vector, Anopheles gambiae. BMC Genomics. 2011; 12: 296 [OpenAIRE] [PubMed]

Giraldo-Calderon, GI. VectorBase: an updated bioinformatics resource for invertebrate vectors and other organisms related with human diseases. Nucleic Acids Res. 2015; 43: D707-713 [OpenAIRE] [PubMed]

Claycomb, JM, Benasutti, M, Bosco, G, Fenger, DD, Orr-Weaver, TL. Gene amplification as a developmental strategy: isolation of two developmental amplicons in Drosophila. Dev Cell. 2004; 6: 145-155 [OpenAIRE] [PubMed]

Hong, CC, Hashimoto, C. The maternal nudel protein of Drosophila has two distinct roles important for embryogenesis. Genetics. 1996; 143: 1653-1661 [OpenAIRE] [PubMed]

Bateman, JR, Lee, AM, Wu, CT. Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Genetics. 2006; 173: 769-777 [OpenAIRE] [PubMed]

31 references, page 1 of 3
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue