publication . Article . 2012

Production of Antifungal Chitinase by Aspergillus niger LOCK 62 and Its Potential Role in the Biological Control

Maria Swiontek Brzezinska; Urszula Jankiewicz;
Open Access English
  • Published: 26 Aug 2012 Journal: Current Microbiology, volume 65, issue 6, pages 666-672 (issn: 0343-8651, Copyright policy)
  • Publisher: Springer Nature
Aspergillus niger LOCK 62 produces an antifungal chitinase. Different sources of chitin in the medium were used to test the production of the chitinase. Chitinase production was most effective when colloidal chitin and shrimp shell were used as substrates. The optimum incubation period for chitinase production by Aspergillus niger LOCK 62 was 6 days. The chitinase was purified from the culture medium by fractionation with ammonium sulfate and affinity chromatography. The molecular mass of the purified enzyme was 43 kDa. The highest activity was obtained at 40 °C for both crude and purified enzymes. The crude chitinase activity was stable during 180 min incubatio...
Medical Subject Headings: food and beveragesfungi
free text keywords: Applied Microbiology and Biotechnology, Microbiology, Article, General Medicine, Biology, Botrytis cinerea, biology.organism_classification, Aspergillus niger, Fusarium oxysporum, Rhizoctonia solani, Food science, Chitin, chemistry.chemical_compound, chemistry, Fusarium solani, Alternaria alternata, Chitinase, biology.protein
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