publication . Article . Other literature type . 1980

Quantitative microtiter cytotoxicity assay for Shigella toxin.

Gentry, M K; Dalrymple, J M;
Open Access
  • Published: 01 Sep 1980 Journal: Journal of Clinical Microbiology, volume 12, pages 361-366 (issn: 0095-1137, eissn: 1098-660X, Copyright policy)
  • Publisher: American Society for Microbiology
Abstract
The cytotoxic activity of Shigella dysenteriae 1 was assayed by exposing HeLa cells in microtiter cultures to dilutions of toxin. Exposure to toxin caused either failure of cells in suspension to attach or detachment of cells from established monolayers. Estimates of toxin potency were made by staining residual cells with crystal violet and visually inspecting the stained plates. Quantitation of the cytotoxic effect was made possible by eluting and spectrophotometrically measuring the stain. The dilution of toxin causing 50% cell detachment, the endpoint chosen for the assay, was estimated from plots of dye absorbance versus toxin dilution. The 50% cell detachme...
Subjects
free text keywords: Microbiology (medical), Microbiology, Toxin, medicine.disease_cause, medicine, Antitoxin, Molecular biology, HeLa, biology.organism_classification, biology, Cytotoxicity, Shigella dysenteriae, Cell culture, Serial dilution, Staining, Research Article
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publication . Article . Other literature type . 1980

Quantitative microtiter cytotoxicity assay for Shigella toxin.

Gentry, M K; Dalrymple, J M;