publication . Article . 2016

Modulation of Human Airway Barrier Functions during Burkholderia thailandensis and Francisella tularensis Infection Running Title: Airway Barrier Functions during Bacterial Infections

Blume, Cornelia; David, Jonathan; Bell, Rachel E.; Laver, Jay R.; Read, Robert C.; Clark, Graeme C.; Davies, Donna E.; Swindle, Emily J.;
Open Access English
  • Published: 03 Aug 2016 Journal: Pathogens (issn: 2076-0817, Copyright policy)
  • Publisher: MDPI AG
  • Country: United States
The bronchial epithelium provides protection against pathogens from the inhaled environment through the formation of a highly-regulated barrier. In order to understand the pulmonary diseases melioidosis and tularemia caused by Burkholderia thailandensis and Fransicella tularensis, respectively, the barrier function of the human bronchial epithelium were analysed. Polarised 16HBE14o- or differentiated primary human bronchial epithelial cells (BECs) were exposed to increasing multiplicities of infection (MOI) of B. thailandensis or F. tularensis Live Vaccine Strain and barrier responses monitored over 24–72 h. Challenge of polarized BECs with either bacterial spec...
free text keywords: airway epithelium, bacterial infection, Burkholderia thailandensis, Fransicella tularensis, barrier functions, bacterial passage, fluticasone propionate, Medicine, R, Article, Virology, Tumor necrosis factor alpha, Biology, Barrier function, biology.organism_classification, Blocking antibody, Respiratory system, Tight junction, Immunology, Microbiology, Respiratory epithelium, Tularemia, medicine.disease
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