publication . Article . 2012

Detection of Viable Mycobacterium ulcerans in Clinical Samples by a Novel Combined 16S rRNA Reverse Transcriptase/IS2404 Real-Time qPCR Assay

Richard Phillips; Yaw A Amoako;
Open Access
  • Published: 28 Aug 2012
  • Publisher: Public Library of Science (PLoS)
Abstract
PLOS Neglected Tropical Diseases 1 August 2012 | Volume 6 | Issue 8 | e1756.Also available at www.plosntds.org
Subjects
free text keywords: Medizin, Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270, From Innovation to Application, Medicine, Infectious Diseases, Bacterial Diseases, Buruli Ulcer, Neglected Tropical Diseases, ddc:610
Funded by
EC| BURULIVAC
Project
BURULIVAC
Identification and development of vaccine candidates for Buruli Ulcer Disease
  • Funder: European Commission (EC)
  • Project Code: 241500
  • Funding stream: FP7 | SP1 | HEALTH
16 references, page 1 of 2

1. World Health Organization (2008) Buruli ulcer: progress report, 2004-2008. Wkly Epidemiol Rec 83: 145-156.

2. World Health Organization (2008) Meeting of the WHO Technical Advisory Group on Buruli ulcer, 3 April 2008, Geneva, summary report. Geneva: World Health Organization.

3. Beissner M, Herbinger KH, Bretzel G (2010) Laboratory diagnosis of Buruli ulcer disease. Future Microbiol 5(3): 363-370. [OpenAIRE]

4. Ruf MT, Chauty A, Adeye A, Ardant MF, Koussemou H, et al. (2011) Secondary Buruli ulcer skin lesions emerging several months after completion of chemotherapy: paradoxical reaction or evidence for immune protection? PLoS Negl Trop Dis 5(8): e1252. doi:10.1371/journal. pntd.0001252

5. Nienhuis WA, Stienstra Y, Abass KM, Tuah W, Thompson WA, et al. (2011) Paradoxical responses after start of antimicrobial treatment in monitoring of treatment success and provides a powerful tool for clinical research. [OpenAIRE]

GenBank Accession Numbers Genes or DNA sequences of mycobacterial strains used in this study were retrieved from GenBank (NCBI) [13].

The respective sequences and accession numbers are summarized in Table S1.

Protocol S1 Preparation of PANTA transport medium and stabilisation of M. Mycobacterium ulcerans infection. Clin Infect Dis 54(4): 519-526.

6. Martinez AN, Lahiri R, Pittman TL, Scollard D, Truman R, et al. (2009) Molecular determination of Mycobacterium leprae viability by use of realtime PCR. J Clin Microbiol 47(7): 2124-2130.

7. Desjardin LE, Perkins MD, Wolski K, Haun S, Teixeira L, et al. (1999) Measurement of sputum Mycobacterium tuberculosis messenger RNA as a surrogate for response to chemotherapy. Am J Respir Crit Care Med 160: 203-210.

8. Bretzel G, Siegmund V, Racz P, van Vloten F, Ngos F, et al. (2005) Post-surgical assessment of excised tissue from patients with Buruli ulcer disease: progression of infection in macroscopically healthy tissue. Trop Med Int Health 10(11): 1199-1206.

9. Fyfe JA, Lavender CJ, Johnson PD, Globan M, Sievers A, et al. (2007) Development and application of two multiplex real-time PCR assays for the detection of Mycobacterium ulcerans in clinical and environmental samples. Appl Environ Microbiol 73(15): 4733-4740.

10. Taylor S, Wakem M, Dijkman G, Alsarraj M, Nguyen M (2010) A practical approach to RTqPCR-Publishing data that conform to the MIQE guidelines. Methods 50(4): S1-S5.

11. Beissner M, Awua-Boateng NY, Thompson W, Nienhuis WA, Klutse E, et al. (2010) A genotypic approach for detection, identification, and characterization of drug resistance in Mycobacterium ulcerans in clinical samples and isolates from Ghana. Am J Trop Med Hyg 83(5): 1059-1065.

12. Talaat AM, Reimschuessel R, Trucksis M. (1997) Identification of mycobacteria infecting fish to the species level using polymerase chain reaction and restriction enzyme analysis. Vet Microbiol 58(2- 4): 229-237.

16 references, page 1 of 2
Abstract
PLOS Neglected Tropical Diseases 1 August 2012 | Volume 6 | Issue 8 | e1756.Also available at www.plosntds.org
Subjects
free text keywords: Medizin, Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270, From Innovation to Application, Medicine, Infectious Diseases, Bacterial Diseases, Buruli Ulcer, Neglected Tropical Diseases, ddc:610
Funded by
EC| BURULIVAC
Project
BURULIVAC
Identification and development of vaccine candidates for Buruli Ulcer Disease
  • Funder: European Commission (EC)
  • Project Code: 241500
  • Funding stream: FP7 | SP1 | HEALTH
16 references, page 1 of 2

1. World Health Organization (2008) Buruli ulcer: progress report, 2004-2008. Wkly Epidemiol Rec 83: 145-156.

2. World Health Organization (2008) Meeting of the WHO Technical Advisory Group on Buruli ulcer, 3 April 2008, Geneva, summary report. Geneva: World Health Organization.

3. Beissner M, Herbinger KH, Bretzel G (2010) Laboratory diagnosis of Buruli ulcer disease. Future Microbiol 5(3): 363-370. [OpenAIRE]

4. Ruf MT, Chauty A, Adeye A, Ardant MF, Koussemou H, et al. (2011) Secondary Buruli ulcer skin lesions emerging several months after completion of chemotherapy: paradoxical reaction or evidence for immune protection? PLoS Negl Trop Dis 5(8): e1252. doi:10.1371/journal. pntd.0001252

5. Nienhuis WA, Stienstra Y, Abass KM, Tuah W, Thompson WA, et al. (2011) Paradoxical responses after start of antimicrobial treatment in monitoring of treatment success and provides a powerful tool for clinical research. [OpenAIRE]

GenBank Accession Numbers Genes or DNA sequences of mycobacterial strains used in this study were retrieved from GenBank (NCBI) [13].

The respective sequences and accession numbers are summarized in Table S1.

Protocol S1 Preparation of PANTA transport medium and stabilisation of M. Mycobacterium ulcerans infection. Clin Infect Dis 54(4): 519-526.

6. Martinez AN, Lahiri R, Pittman TL, Scollard D, Truman R, et al. (2009) Molecular determination of Mycobacterium leprae viability by use of realtime PCR. J Clin Microbiol 47(7): 2124-2130.

7. Desjardin LE, Perkins MD, Wolski K, Haun S, Teixeira L, et al. (1999) Measurement of sputum Mycobacterium tuberculosis messenger RNA as a surrogate for response to chemotherapy. Am J Respir Crit Care Med 160: 203-210.

8. Bretzel G, Siegmund V, Racz P, van Vloten F, Ngos F, et al. (2005) Post-surgical assessment of excised tissue from patients with Buruli ulcer disease: progression of infection in macroscopically healthy tissue. Trop Med Int Health 10(11): 1199-1206.

9. Fyfe JA, Lavender CJ, Johnson PD, Globan M, Sievers A, et al. (2007) Development and application of two multiplex real-time PCR assays for the detection of Mycobacterium ulcerans in clinical and environmental samples. Appl Environ Microbiol 73(15): 4733-4740.

10. Taylor S, Wakem M, Dijkman G, Alsarraj M, Nguyen M (2010) A practical approach to RTqPCR-Publishing data that conform to the MIQE guidelines. Methods 50(4): S1-S5.

11. Beissner M, Awua-Boateng NY, Thompson W, Nienhuis WA, Klutse E, et al. (2010) A genotypic approach for detection, identification, and characterization of drug resistance in Mycobacterium ulcerans in clinical samples and isolates from Ghana. Am J Trop Med Hyg 83(5): 1059-1065.

12. Talaat AM, Reimschuessel R, Trucksis M. (1997) Identification of mycobacteria infecting fish to the species level using polymerase chain reaction and restriction enzyme analysis. Vet Microbiol 58(2- 4): 229-237.

16 references, page 1 of 2
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