publication . Article . Other literature type . 1984

Isolation and characterization of the outer membrane and lipopolysaccharide from Eikenella corrodens.

Progulske, A; Holt, S C;
Open Access
  • Published: 01 Jan 1984 Journal: Infection and Immunity, volume 43, pages 166-177 (issn: 0019-9567, eissn: 1098-5522, Copyright policy)
  • Publisher: American Society for Microbiology
Abstract
The chemical composition of the outer membrane fractions (OMFs) of Eikenella corrodens strains 23834 and 470 as well as the strain 23834 lipopolysaccharide (LPS) was determined. The OMFs were obtained by Triton X-100 treatment of the heavier membrane fraction from sucrose density centrifugation of the total membrane fraction. The resulting OMFs of strains 23834 and 470, free of cytoplasmic membrane components, were found to contain 69.6 and 75.0% (wt/wt) protein, 4.8 and 9.2% lipid, 4.6 and 4.7% carbohydrate, and 2.0 and 4.6% muramic acid, respectively. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis both OMFs contained one major peptide determined ...
Subjects
free text keywords: Immunology, Microbiology, Parasitology, Infectious Diseases, Biochemistry, Muramic acid, chemistry.chemical_compound, chemistry, Fatty acid, chemistry.chemical_classification, Eikenella corrodens, biology.organism_classification, biology, Heptose, Octadecenoic Acid, Membrane lipids, Gel electrophoresis, Bacterial outer membrane, Research Article
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publication . Article . Other literature type . 1984

Isolation and characterization of the outer membrane and lipopolysaccharide from Eikenella corrodens.

Progulske, A; Holt, S C;