publication . Article . 1998

When Overexpressed, a Novel Centrosomal Protein, RanBPM, Causes Ectopic Microtubule Nucleation Similar to γ-Tubulin

Masafumi Nakamura; Hirohisa Masuda; Johji Horii; Kei-ichi Kuma; Nobuhiko Yokoyama; Tomoyuki Ohba; Hideo Nishitani; Takashi Miyata; Masao Tanaka; Takeharu Nishimoto;
Open Access English
  • Published: 16 Nov 1998 Journal: The Journal of Cell Biology, volume 143, issue 4, pages 1,041-1,052 (issn: 0021-9525, eissn: 1540-8140, Copyright policy)
  • Publisher: The Rockefeller University Press
Abstract
<jats:p>A novel human protein with a molecular mass of 55 kD, designated RanBPM, was isolated with the two-hybrid method using Ran as a bait. Mouse and hamster RanBPM possessed a polypeptide identical to the human one. Furthermore, Saccharomyces cerevisiae was found to have a gene, YGL227w, the COOH-terminal half of which is 30% identical to RanBPM. Anti-RanBPM antibodies revealed that RanBPM was localized within the centrosome throughout the cell cycle. Overexpression of RanBPM produced multiple spots which were colocalized with γ-tubulin and acted as ectopic microtubule nucleation sites, resulting in a reorganization of microtubule network. RanBPM cosedimented...
Subjects
free text keywords: Regular Articles, centrosome, γ-tubulin, Ran, RanBPM, YGL227w, Cell Biology, Tubulin, biology.protein, biology, Cytoskeleton, Microtubule, Molecular biology, GTP-binding protein regulators, Nuclear protein, Microtubule nucleation
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