publication . Other literature type . Article . 2013

Development of a methodology for and assessment of pulsatile luteinizing hormone secretion in juvenile and adult male mice.

Steyn, F. J.; Wan, Y.; Clarkson, J.; Veldhuis, J. D.; Herbison, A. E.; Chen, C.;
Open Access English
  • Published: 03 Oct 2013
  • Publisher: Endocrine Society
Abstract
Current methodology to monitor pulsatile LH release in mice is limited by inadequate assay sensitivity, resulting in the need for collection of large blood volumes. Thus, assessment of pulsatile LH secretion in mice remains highly challenging, and observations are limited to adult mice. To address this, we developed a highly sensitive ELISA for assessment of mouse LH concentrations in small fractions of whole blood. We demonstrate that this assay is capable of reliably detecting LH down to a theoretical limit of 0.117 ng/mL in a 2-μL fraction of whole blood. Using an established frequent blood collection procedure, we validated the accuracy of this method by det...
Subjects
free text keywords: Technical Communication, Endocrinology, Assay sensitivity, medicine.medical_specialty, medicine, Biology, Pulsatile flow, Luteinizing hormone secretion, Luteinizing hormone, Secretion, Internal medicine, Whole blood, Knockout mouse, Diabetes mellitus, medicine.disease
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publication . Other literature type . Article . 2013

Development of a methodology for and assessment of pulsatile luteinizing hormone secretion in juvenile and adult male mice.

Steyn, F. J.; Wan, Y.; Clarkson, J.; Veldhuis, J. D.; Herbison, A. E.; Chen, C.;