publication . Article . Other literature type . 1993

Interaction forces between red cells agglutinated by antibody. IV. Time and force dependence of break-up

David F. J. Tees; Harry L. Goldsmith; O. Coenen;
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  • Published: 01 Sep 1993 Journal: Biophysical Journal, volume 65, pages 1,318-1,334 (issn: 0006-3495, Copyright policy)
  • Publisher: Elsevier BV
Abstract
We report on an extension of a previously described method to measure the hydrodynamic force to separate doublets of fixed, sphered and swollen red cells cross-linked by antibody (S. P. Tha, J. Shuster, and H. L. Goldsmith. 1986. Biophys. J. 50:1117-1126). With a traveling microtube apparatus, doublets are tracked and videotaped in a slowly accelerating Poiseuille flow in 150-microns-diameter tubes, and the hydrodynamic normal force at break-up, Fn, is computed from the measured doublet velocity and radial position. Previous results showed a large range of Fn, the mean of which increased with [antiserum], and an absence of clustering at discrete values of Fn. Si...
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doi: 10.1016/s0006-3495(93)81180-9
pmc: PMC1225851
pmid: 8241411
Subjects
free text keywords: Biophysics, Research Article, Molecular physics, Break-Up, Couette flow, Stereochemistry, Normal force, Rheoscope, Hagen–Poiseuille equation, Antibody, biology.protein, biology, Shear (sheet metal), Chemistry, Shear stress
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Other literature type . 1993
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