publication . Article . Other literature type . 2003

A proteomics approach to understanding protein ubiquitination.

Peng, Junmin; Schwartz, Daniel; Elias, Joshua E; Thoreen, Carson C; Cheng, Dongmei; Marsischky, Gerald; Roelofs, Jeroen; Finley, Daniel; Gygi, Steven P;
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  • Published: 20 Jul 2003 Journal: Nature Biotechnology, volume 21, pages 921-926 (issn: 1087-0156, eissn: 1546-1696, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
Abstract
There is a growing need for techniques that can identify and characterize protein modifications on a large or global scale. We report here a proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate. Ubiquitin conjugates from a strain expressing 6xHis-tagged ubiquitin were isolated, proteolyzed with trypsin and analyzed by multidimensional liquid chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for amino acid sequence determination. We identified 1,075 proteins from the sample. In addition, we detected 110 precise ubiquitination sites present in 72 ubiquitin-protein conjugates. Finally, ubiqu...
Subjects
free text keywords: Ubiquitin, biology.protein, biology, Protein ubiquitination, Proteomics, Tandem mass spectrometry, Ubiquitin ligase, Lysine, Peptide sequence, Biochemistry, Ubiquitin-conjugating enzyme
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