publication . Article . Other literature type . 2013

Metagenome and metaproteome analyses of microbial communities in mesophilic biogas-producing anaerobic batch fermentations indicate concerted plant carbohydrate degradation.

Dirk Benndorf; Ulrike Schimpf; Martha Zakrzewski; Angelika Hanreich; Alfred Pühler; Erdmann Rapp; Andreas Schlüter; Michael Klocke; Robert Heyer; Udo Reichl;
Restricted English
  • Published: 01 Jul 2013 Journal: Systematic and Applied Microbiology, volume 36, issue 5 (issn: 0723-2020, Copyright policy)
  • Publisher: Elsevier BV
  • Country: Germany
Abstract
Microbial communities in biogas batch fermentations, using straw and hay as co-substrates, were analyzed at the gene and protein level by metagenomic and metaproteomic approaches. The analysis of metagenomic data revealed that the Clostridiales and Bacteroidales orders were prevalent in the community. However, the number of sequences assigned to the Clostridiales order decreased during fermentation, whereas the number of sequences assigned to the Bacteroidales order increased. In addition, changes at the functional level were monitored and the metaproteomic analyses detected transporter proteins and flagellins, which were expressed mainly by members of the Bacte...
Subjects
free text keywords: Applied Microbiology and Biotechnology, Ecology, Evolution, Behavior and Systematics, Microbiology, Biochemistry, Firmicutes, biology.organism_classification, biology, Bacteroidetes, Clostridiales, Methanogenesis, Bacteroidales, Phylum, Fermentation, Metagenomics
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