publication . Article . 2006

Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123)

Bjarne Due Larsen; Martin Stahlhut; James K. Hennan; Anne Louise Kjølbye; N. H. Holstein-Rathlou; Shabaz Mohammed; Morten Schak Nielsen; Søren Andersen; Ole Nørregaard Jensen; Lene Nygaard Axelsen;
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  • Published: 01 Jun 2006
  • Publisher: Elsevier BV
Abstract
Previous studies suggest that dephosphorylation of connexin43 (Cx43) is related to uncoupling of gap junction communication, which plays an important role in the genesis of ischemia-induced ventricular tachycardia. We studied changes in Cx43 phosphorylation during global ischemia in the absence and presence of the antiarrhythmic peptide analogue rotigaptide (formerly known as ZP123). Phosphorylation analysis was performed on Cx43 purified from isolated perfused rat hearts using matrix-assisted laser desorption/ionization mass spectrometry and liquid chromatography electrospray ionization tandem mass spectrometry. Thirteen different serine phosphorylation sites w...
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Subjects
Medical Subject Headings: cardiovascular system
free text keywords: Molecular Biology, Cardiology and Cardiovascular Medicine, Ischemia, medicine.disease, medicine, Rotigaptide, chemistry.chemical_compound, chemistry, Pharmacology, Serine, Dephosphorylation, PMSF, Phosphorylation, Gating, Gap junction, Biochemistry
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Abstract
Previous studies suggest that dephosphorylation of connexin43 (Cx43) is related to uncoupling of gap junction communication, which plays an important role in the genesis of ischemia-induced ventricular tachycardia. We studied changes in Cx43 phosphorylation during global ischemia in the absence and presence of the antiarrhythmic peptide analogue rotigaptide (formerly known as ZP123). Phosphorylation analysis was performed on Cx43 purified from isolated perfused rat hearts using matrix-assisted laser desorption/ionization mass spectrometry and liquid chromatography electrospray ionization tandem mass spectrometry. Thirteen different serine phosphorylation sites w...
Persistent Identifiers
Subjects
Medical Subject Headings: cardiovascular system
free text keywords: Molecular Biology, Cardiology and Cardiovascular Medicine, Ischemia, medicine.disease, medicine, Rotigaptide, chemistry.chemical_compound, chemistry, Pharmacology, Serine, Dephosphorylation, PMSF, Phosphorylation, Gating, Gap junction, Biochemistry
Related research
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