publication . Article . 2008

Lipopolysaccharide plus hypoxia and reoxygenation synergistically reduce electrical coupling between microvascular endothelial cells by dephosphorylating connexin40.

Michael L. Bolon; Michael L. Bolon; Gerald M. Kidder; Gerald M. Kidder; Tianqing Peng; Tianqing Peng; Karel Tyml; Karel Tyml;
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  • Published: 02 Jun 2008
Abstract
We showed that lipopolysaccharide (LPS) or hypoxia and reoxygenation (H/R) decreases electrical coupling between microvascular endothelial cells by targeting the gap junction protein connexin40 (Cx40), tyrosine kinase-, ERK1/2-, and PKA-dependently. Since LPS can compromise microvascular blood flow, resulting in micro-regional H/R, the concurrent LPS + H/R could reduce coupling to a much greater extent than LPS or H/R alone. We examined this possibility in a model of cultured microvascular endothelial cells (mouse skeletal muscle origin) in terms of electrical coupling and the phosphorylation status of Cx40. To assess coupling, we measured the spread of electric...
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free text keywords: Clinical Biochemistry, Cell Biology, Physiology, Serine, Tyrosine kinase, Wild type, Lipopolysaccharide, chemistry.chemical_compound, chemistry, Null cell, Phosphorylation, Biology, Skeletal muscle, medicine.anatomical_structure, medicine, Molecular biology, Intracellular, Biochemistry
Related research
Abstract
We showed that lipopolysaccharide (LPS) or hypoxia and reoxygenation (H/R) decreases electrical coupling between microvascular endothelial cells by targeting the gap junction protein connexin40 (Cx40), tyrosine kinase-, ERK1/2-, and PKA-dependently. Since LPS can compromise microvascular blood flow, resulting in micro-regional H/R, the concurrent LPS + H/R could reduce coupling to a much greater extent than LPS or H/R alone. We examined this possibility in a model of cultured microvascular endothelial cells (mouse skeletal muscle origin) in terms of electrical coupling and the phosphorylation status of Cx40. To assess coupling, we measured the spread of electric...
Persistent Identifiers
Subjects
free text keywords: Clinical Biochemistry, Cell Biology, Physiology, Serine, Tyrosine kinase, Wild type, Lipopolysaccharide, chemistry.chemical_compound, chemistry, Null cell, Phosphorylation, Biology, Skeletal muscle, medicine.anatomical_structure, medicine, Molecular biology, Intracellular, Biochemistry
Related research
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