publication . Other literature type . Article . 2005

Passive All-Optical Force Clamp for High-Resolution Laser Trapping

Steven M. Block; William J. Greenleaf; Michael T. Woodside; Elio A. Abbondanzieri;
Open Access English
  • Published: 08 Nov 2005
Abstract
Optical traps are useful for studying the effects of forces on single molecules. Feedback-based force clamps are often used to maintain a constant load, but the response time of the feedback limits bandwidth and can introduce instability. We developed a novel force clamp that operates without feedback, taking advantage of the anharmonic region of the trapping potential where the differential stiffness vanishes. We demonstrate the utility of such a force clamp by measuring the unfolding of DNA hairpins and the effect of trap stiffness on opening distance and transition rates.
Subjects
arXiv: Quantitative Biology::Biomolecules
free text keywords: Article, General Physics and Astronomy, Optical tweezers, Trapping, Anharmonicity, Materials science, Nanotechnology, Instability, Response time, Laser, law.invention, law, Clamp, Mechanics, Stiffness, medicine.symptom, medicine
Related Organizations
Any information missing or wrong?Report an Issue