publication . Other literature type . Article . 1976

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Nasser Abbas;
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  • Published: 01 May 1976
  • Publisher: Elsevier BV
Abstract
Abstract A protein determination method which involves the binding of Coomassie Brilliant Blue G-250 to protein is described. The binding of the dye to protein causes a shift in the absorption maximum of the dye from 465 to 595 nm, and it is the increase in absorption at 595 nm which is monitored. This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr. There is little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose. A small amount of color is developed in the presence of strongly alkaline buffering agents, but the assay ...
Subjects
free text keywords: Biophysics, Cell Biology, Biochemistry, Molecular Biology
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