publication . Other literature type . Article . 1988

Partial purification of a bacterial lectinlike substance from Eikenella corrodens.

Y Yamazaki; S Ebisu; H Okada;
Open Access English
  • Published: 01 Jan 1988
Abstract
A bacterial lectinlike substance, which is considered to participate in the adherence of Eikenella corrodens to various host cells, was purified from E. corrodens cells. The substance was extracted in 1% Triton X-100 with sonication from the cell envelope of E. corrodens 1073 and partially purified by galactosamine affinity chromatography and gel filtration chromatography based on its hemagglutination (HA) activity. The lectinlike substance was purified about 256-fold as evaluated by its specific HA activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the partially purified lectinlike substance (PPL) produced a single protein band of large mole...
Subjects
free text keywords: Research Article, Immunology, Microbiology, Parasitology, Infectious Diseases, Muramic acid, chemistry.chemical_compound, chemistry, Glucosamine, Biochemistry, Size-exclusion chromatography, Hemagglutination, Affinity chromatography, Galactosamine, Eikenella corrodens, biology.organism_classification, biology, Gel electrophoresis
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publication . Other literature type . Article . 1988

Partial purification of a bacterial lectinlike substance from Eikenella corrodens.

Y Yamazaki; S Ebisu; H Okada;