publication . Other literature type . Article . 2016

Ultrasound Molecular Imaging of the Breast Cancer Neovasculature using Engineered Fibronectin Scaffold Ligands: A Novel Class of Targeted Contrast Ultrasound Agent.

Abou-Elkacem, Lotfi; Wilson, Katheryne E.; Johnson, Sadie M.; Chowdhury, Sayan M.; Bachawal, Sunitha; Hackel, Benjamin J.; Tian, Lu; Willmann, Jürgen K.;
  • Published: 01 Jun 2016
  • Publisher: Ivyspring International Publisher
Abstract
Molecularly-targeted microbubbles (MBs) are increasingly being recognized as promising contrast agents for oncological molecular imaging with ultrasound. With the detection and validation of new molecular imaging targets, novel binding ligands are needed that bind to molecular imaging targets with high affinity and specificity. In this study we assessed a novel class of potentially clinically translatable MBs using an engineered 10(th) type III domain of human-fibronectin (MB-FN3VEGFR2) scaffold-ligand to image VEGFR2 on the neovasculature of cancer. The in vitro binding of MB-FN3VEGFR2 to a soluble VEGFR2 was assessed by flow-cytometry (FACS) and binding to VEG...
Subjects
free text keywords: Breast cancer, medicine.disease, medicine, Ex vivo, Cancer, Biology, Molecular biology, In vitro, In vivo, Microbubbles, Molecular imaging, Fibronectin, biology.protein, Research Paper, Ultrasound molecular imaging, breast cancer, vascular endothelial growth factor receptor type 2, fibronectin scaffold, protein engineering, yeast display.
Funded by
NIH| Quantification and monitoring inflammation in IBD with Molecular Ultrasound
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 4R01DK092509-05
  • Funding stream: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
,
NIH| Molecular Ultrasound for Early Breast Cancer Detection
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5R01CA155289-04
  • Funding stream: NATIONAL CANCER INSTITUTE
63 references, page 1 of 5

Kiessling, F, Fokong, S, Bzyl, J, Lederle, W, Palmowski, M, Lammers, T. Recent advances in molecular, multimodal and theranostic ultrasound imaging. Adv Drug Deliv Rev. 2014; 72: 15-27 [OpenAIRE] [PubMed]

Klibanov, AL, Hossack, JA. Ultrasound in Radiology: From Anatomic, Functional, Molecular Imaging to Drug Delivery and Image-Guided Therapy. Invest Radiol. 2015; 50: 657-70 [OpenAIRE] [PubMed]

Abou-Elkacem, L, Bachawal, SV, Willmann, JK. Ultrasound molecular imaging: Moving toward clinical translation. Eur J Radiol. 2015; 84: 1685-93 [OpenAIRE] [PubMed]

Pochon, S, Tardy, I, Bussat, P, Bettinger, T, Brochot, J, von Wronski, M. BR55: a lipopeptide-based VEGFR2-targeted ultrasound contrast agent for molecular imaging of angiogenesis. Invest Radiol. 2010; 45: 89-95 [OpenAIRE] [PubMed]

Lyshchik, A, Fleischer, AC, Huamani, J, Hallahan, DE, Brissova, M, Gore, JC. Molecular imaging of vascular endothelial growth factor receptor 2 expression using targeted contrast-enhanced high-frequency ultrasonography. J Ultrasound Med. 2007; 26: 1575-86 [OpenAIRE] [PubMed]

Monsky, WL, Mouta Carreira, C, Tsuzuki, Y, Gohongi, T, Fukumura, D, Jain, RK. Role of host microenvironment in angiogenesis and microvascular functions in human breast cancer xenografts: mammary fat pad versus cranial tumors. Clin Cancer Res. 2002; 8: 1008-13 [PubMed]

Bzyl, J, Lederle, W, Palmowski, M, Kiessling, F. Molecular and functional ultrasound imaging of breast tumors. Eur J Radiol. 2012; 81 (Suppl 1): S11-2 [PubMed]

Bachawal, SV, Jensen, KC, Lutz, AM, Gambhir, SS, Tranquart, F, Tian, L. Earlier detection of breast cancer with ultrasound molecular imaging in a transgenic mouse model. Cancer Res. 2013; 73: 1689-98 [OpenAIRE] [PubMed]

Foygel, K, Wang, H, Machtaler, S, Lutz, AM, Chen, R, Pysz, M. Detection of pancreatic ductal adenocarcinoma in mice by ultrasound imaging of thymocyte differentiation antigen 1. Gastroenterology. 2013; 145: 885-94 [OpenAIRE] [PubMed]

Bachawal, SV, Jensen, KC, Wilson, KE, Tian, L, Lutz, AM, Willmann, JK. Breast Cancer Detection by B7-H3-Targeted Ultrasound Molecular Imaging. Cancer Res. 2015; 75: 2501-9 [OpenAIRE] [PubMed]

Tami, JA, Parr, MD, Brown, SA, Thompson, JS. Monoclonal antibody technology. Am J Hosp Pharm. 1986; 43: 2816-25 [OpenAIRE] [PubMed]

Shukla, AA, Thommes, J. Recent advances in large-scale production of monoclonal antibodies and related proteins. Trends Biotechnol. 2010; 28: 253-61 [OpenAIRE] [PubMed]

Skerra, A. Alternative non-antibody scaffolds for molecular recognition. Curr Opin Biotechnol. 2007; 18: 295-304 [OpenAIRE] [PubMed]

Willmann, JK, Kimura, RH, Deshpande, N, Lutz, AM, Cochran, JR, Gambhir, SS. Targeted contrast-enhanced ultrasound imaging of tumor angiogenesis with contrast microbubbles conjugated to integrin-binding knottin peptides. J Nucl Med. 2010; 51: 433-40 [OpenAIRE] [PubMed]

Wang, X, Hagemeyer, CE, Hohmann, JD, Leitner, E, Armstrong, PC, Jia, F. Novel single-chain antibody-targeted microbubbles for molecular ultrasound imaging of thrombosis: validation of a unique noninvasive method for rapid and sensitive detection of thrombi and monitoring of success or failure of thrombolysis in mice. Circulation. 2012; 125: 3117-26 [OpenAIRE] [PubMed]

63 references, page 1 of 5
Abstract
Molecularly-targeted microbubbles (MBs) are increasingly being recognized as promising contrast agents for oncological molecular imaging with ultrasound. With the detection and validation of new molecular imaging targets, novel binding ligands are needed that bind to molecular imaging targets with high affinity and specificity. In this study we assessed a novel class of potentially clinically translatable MBs using an engineered 10(th) type III domain of human-fibronectin (MB-FN3VEGFR2) scaffold-ligand to image VEGFR2 on the neovasculature of cancer. The in vitro binding of MB-FN3VEGFR2 to a soluble VEGFR2 was assessed by flow-cytometry (FACS) and binding to VEG...
Subjects
free text keywords: Breast cancer, medicine.disease, medicine, Ex vivo, Cancer, Biology, Molecular biology, In vitro, In vivo, Microbubbles, Molecular imaging, Fibronectin, biology.protein, Research Paper, Ultrasound molecular imaging, breast cancer, vascular endothelial growth factor receptor type 2, fibronectin scaffold, protein engineering, yeast display.
Funded by
NIH| Quantification and monitoring inflammation in IBD with Molecular Ultrasound
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 4R01DK092509-05
  • Funding stream: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
,
NIH| Molecular Ultrasound for Early Breast Cancer Detection
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5R01CA155289-04
  • Funding stream: NATIONAL CANCER INSTITUTE
63 references, page 1 of 5

Kiessling, F, Fokong, S, Bzyl, J, Lederle, W, Palmowski, M, Lammers, T. Recent advances in molecular, multimodal and theranostic ultrasound imaging. Adv Drug Deliv Rev. 2014; 72: 15-27 [OpenAIRE] [PubMed]

Klibanov, AL, Hossack, JA. Ultrasound in Radiology: From Anatomic, Functional, Molecular Imaging to Drug Delivery and Image-Guided Therapy. Invest Radiol. 2015; 50: 657-70 [OpenAIRE] [PubMed]

Abou-Elkacem, L, Bachawal, SV, Willmann, JK. Ultrasound molecular imaging: Moving toward clinical translation. Eur J Radiol. 2015; 84: 1685-93 [OpenAIRE] [PubMed]

Pochon, S, Tardy, I, Bussat, P, Bettinger, T, Brochot, J, von Wronski, M. BR55: a lipopeptide-based VEGFR2-targeted ultrasound contrast agent for molecular imaging of angiogenesis. Invest Radiol. 2010; 45: 89-95 [OpenAIRE] [PubMed]

Lyshchik, A, Fleischer, AC, Huamani, J, Hallahan, DE, Brissova, M, Gore, JC. Molecular imaging of vascular endothelial growth factor receptor 2 expression using targeted contrast-enhanced high-frequency ultrasonography. J Ultrasound Med. 2007; 26: 1575-86 [OpenAIRE] [PubMed]

Monsky, WL, Mouta Carreira, C, Tsuzuki, Y, Gohongi, T, Fukumura, D, Jain, RK. Role of host microenvironment in angiogenesis and microvascular functions in human breast cancer xenografts: mammary fat pad versus cranial tumors. Clin Cancer Res. 2002; 8: 1008-13 [PubMed]

Bzyl, J, Lederle, W, Palmowski, M, Kiessling, F. Molecular and functional ultrasound imaging of breast tumors. Eur J Radiol. 2012; 81 (Suppl 1): S11-2 [PubMed]

Bachawal, SV, Jensen, KC, Lutz, AM, Gambhir, SS, Tranquart, F, Tian, L. Earlier detection of breast cancer with ultrasound molecular imaging in a transgenic mouse model. Cancer Res. 2013; 73: 1689-98 [OpenAIRE] [PubMed]

Foygel, K, Wang, H, Machtaler, S, Lutz, AM, Chen, R, Pysz, M. Detection of pancreatic ductal adenocarcinoma in mice by ultrasound imaging of thymocyte differentiation antigen 1. Gastroenterology. 2013; 145: 885-94 [OpenAIRE] [PubMed]

Bachawal, SV, Jensen, KC, Wilson, KE, Tian, L, Lutz, AM, Willmann, JK. Breast Cancer Detection by B7-H3-Targeted Ultrasound Molecular Imaging. Cancer Res. 2015; 75: 2501-9 [OpenAIRE] [PubMed]

Tami, JA, Parr, MD, Brown, SA, Thompson, JS. Monoclonal antibody technology. Am J Hosp Pharm. 1986; 43: 2816-25 [OpenAIRE] [PubMed]

Shukla, AA, Thommes, J. Recent advances in large-scale production of monoclonal antibodies and related proteins. Trends Biotechnol. 2010; 28: 253-61 [OpenAIRE] [PubMed]

Skerra, A. Alternative non-antibody scaffolds for molecular recognition. Curr Opin Biotechnol. 2007; 18: 295-304 [OpenAIRE] [PubMed]

Willmann, JK, Kimura, RH, Deshpande, N, Lutz, AM, Cochran, JR, Gambhir, SS. Targeted contrast-enhanced ultrasound imaging of tumor angiogenesis with contrast microbubbles conjugated to integrin-binding knottin peptides. J Nucl Med. 2010; 51: 433-40 [OpenAIRE] [PubMed]

Wang, X, Hagemeyer, CE, Hohmann, JD, Leitner, E, Armstrong, PC, Jia, F. Novel single-chain antibody-targeted microbubbles for molecular ultrasound imaging of thrombosis: validation of a unique noninvasive method for rapid and sensitive detection of thrombi and monitoring of success or failure of thrombolysis in mice. Circulation. 2012; 125: 3117-26 [OpenAIRE] [PubMed]

63 references, page 1 of 5
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