publication . Other literature type . Article . 2015

Deacetylation of Nuclear LC3 Drives Autophagy Initiation under Starvation

Huang, Rui; Xu, Yinfeng; Wan, Wei; Shou, Xin; Qian, Jiali; You, Zhiyuan; Liu, Bo; Chang, Chunmei; Zhou, Tianhua; Lippincott-Schwartz, Jennifer; ...
  • Published: 01 Feb 2015
  • Publisher: Elsevier BV
Abstract
Summary Shuttling of macromolecules between different cellular compartments helps regulate the timing and extent of different cellular activities. Here, we report that LC3, a key initiator of autophagy that cycles between the nucleus and cytoplasm, becomes selectively activated in the nucleus during starvation through deacetylation by the nuclear deacetylase Sirt1. Deacetylation of LC3 at K49 and K51 by Sirt1 allows LC3 to interact with the nuclear protein DOR and return to the cytoplasm with DOR, where it is able to bind Atg7 and other autophagy factors and undergo phosphatidylethanolamine conjugation to preautophagic membranes. The association of deacetylated ...
Subjects
Medical Subject Headings: biological phenomena, cell phenomena, and immunityembryonic structures
free text keywords: Genetics, Autophagy, Cell nucleus, medicine.anatomical_structure, medicine, Cell biology, Acetylation, Nuclear protein, Nucleus, Cytoplasm, Biology, Cell, Autophagy-Related Protein 7, Molecular Biology
Related Organizations
Abstract
Summary Shuttling of macromolecules between different cellular compartments helps regulate the timing and extent of different cellular activities. Here, we report that LC3, a key initiator of autophagy that cycles between the nucleus and cytoplasm, becomes selectively activated in the nucleus during starvation through deacetylation by the nuclear deacetylase Sirt1. Deacetylation of LC3 at K49 and K51 by Sirt1 allows LC3 to interact with the nuclear protein DOR and return to the cytoplasm with DOR, where it is able to bind Atg7 and other autophagy factors and undergo phosphatidylethanolamine conjugation to preautophagic membranes. The association of deacetylated ...
Subjects
Medical Subject Headings: biological phenomena, cell phenomena, and immunityembryonic structures
free text keywords: Genetics, Autophagy, Cell nucleus, medicine.anatomical_structure, medicine, Cell biology, Acetylation, Nuclear protein, Nucleus, Cytoplasm, Biology, Cell, Autophagy-Related Protein 7, Molecular Biology
Related Organizations
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