publication . Other literature type . Article . 2008

Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry.

Meierhofer, D.; Wang, X.; Huang, L.; Kaiser, P.;
  • Published: 03 Oct 2008
  • Publisher: American Chemical Society (ACS)
Abstract
Ubiquitination regulates a host of cellular processes by labeling proteins for degradation, but also by functioning as a regulatory, nonproteolytic posttranslational modification. Proteome-wide strategies to monitor changes in ubiquitination profiles are important to obtain insight into the various cellular functions of ubiquitination. Here we describe generation of stable cell lines expressing a tandem hexahistidine-biotin tag (HB-tag) fused to ubiquitin for two-step purification of the ubiquitinated proteome under fully denaturing conditions. Using this approach we identified 669 ubiquitinated proteins from HeLa cells, including 44 precise ubiquitin attachment...
Subjects
free text keywords: Article, Biochemistry, General Chemistry, Stable isotope labeling by amino acids in cell culture, F-box protein, biology.protein, biology, Proteasome inhibitor, medicine.drug, medicine, Molecular biology, Proteasome, MG132, chemistry.chemical_compound, chemistry, Ubiquitin-conjugating enzyme, Ubiquitin, Ubiquitin ligase
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