publication . Other literature type . Article . 2006

Cotranscriptional coupling of splicing factor recruitment and precursor messenger RNA splicing in mammalian cells.

Imke, Listerman; Aparna K, Sapra; Karla M, Neugebauer;
  • Published: 20 Aug 2006
  • Publisher: Springer Science and Business Media LLC
Coupling between transcription and RNA processing is a key gene regulatory mechanism. Here we use chromatin immunoprecipitation to detect transcription-dependent accumulation of the precursor mRNA (pre-mRNA) splicing factors hnRNP A1, U2AF65 and U1 and U5 snRNPs on the intron-containing human FOS gene. These factors were poorly detected on intronless heat-shock and histone genes, a result that opposes direct recruitment by RNA polymerase II (Pol II) or the cap-binding complex in vivo. However, an observed RNA-dependent interaction between U2AF65 and active forms of Pol II may stabilize U2AF65 binding to intron-containing nascent RNA. We establish chromatin-RNA i...
free text keywords: Molecular Biology, Structural Biology
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