publication . Article . Other literature type . 2012

Rapid, Field-Deployable Method for Genotyping and Discovery of Single-Nucleotide Polymorphisms Associated with Drug Resistance in Plasmodium falciparum

Jason T. Mckinney; Sarah K. Volkman; Sarah K. Volkman; Mikeal Wall; Dyann F. Wirth; Daouda Ndiaye; Souleymane Mboup; Rachel F. Daniels; P.D. Sène; Pardis C. Sabeti;
Open Access English
  • Published: 19 Mar 2012
  • Publisher: American Society for Microbiology
  • Country: United States
Abstract
Despite efforts to reduce malaria morbidity and mortality, drug-resistant parasites continue to evade control strategies. Recently, emphasis has shifted away from control and toward regional elimination and global eradication of malaria. Such a campaign requires tools to monitor genetic changes in the parasite that could compromise the effectiveness of antimalarial drugs and undermine eradication programs. These tools must be fast, sensitive, unambiguous, and cost-effective to offer real-time reports of parasite drug susceptibility status across the globe. We have developed and validated a set of genotyping assays using high-resolution melting (HRM) analysis to ...
Subjects
free text keywords: antimalarials, pharmacology, drug resistance, genetics, mutation, parasitic sensitivity traits, Plasmodium falciparum, single nucleotide polymorphism, Pharmacology (medical), Infectious Diseases, Analytical Procedures, Genotype, Malaria, medicine.disease, medicine, Locus (genetics), Biology, biology.organism_classification, Single-nucleotide polymorphism, Genotyping, medicine.disease_cause
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