publication . Article . Other literature type . 2012

Rapid, Field-Deployable Method for Genotyping and Discovery of Single-Nucleotide Polymorphisms Associated with Drug Resistance in Plasmodium falciparum

Name: Rapid, Field-Deployable Method for Genotyping and Discovery of Single-Nucleotide Polymorphisms Associated with Drug Resistance in Plasmodium falciparum
Keywords: antimalarials, pharmacology, drug resistance, genetics, mutation, parasitic sensitivity traits, Plasmodium falciparum, single nucleotide polymorphism, Pharmacology (medical), Infectious Diseases, Analytical Procedures

Jason T. Mckinney; Sarah K. Volkman; Sarah K. Volkman; Mikeal Wall; Dyann F. Wirth; Daouda Ndiaye; Souleymane Mboup; Rachel F. Daniels; P.D. Sène; Pardis C. Sabeti;

Open Access English

Published: 19 Mar 2012

Publisher: American Society for Microbiology

Country: United States

Summary
Related research

Abstract

Despite efforts to reduce malaria morbidity and mortality, drug-resistant parasites continue to evade control strategies. Recently, emphasis has shifted away from control and toward regional elimination and global eradication of malaria. Such a campaign requires tools to monitor genetic changes in the parasite that could compromise the effectiveness of antimalarial drugs and undermine eradication programs. These tools must be fast, sensitive, unambiguous, and cost-effective to offer real-time reports of parasite drug susceptibility status across the globe. We have developed and validated a set of genotyping assays using high-resolution melting (HRM) analysis to ...

doi: 10.1128/aac.05737-11

pmid: 22430961

pmc: PMC3370755

Subjects

free text keywords: antimalarials, pharmacology, drug resistance, genetics, mutation, parasitic sensitivity traits, Plasmodium falciparum, single nucleotide polymorphism, Pharmacology (medical), Infectious Diseases, Analytical Procedures, Genotype, Malaria, medicine.disease, medicine, Locus (genetics), Biology, biology.organism_classification, Single-nucleotide polymorphism, Genotyping, medicine.disease_cause

Related Organizations