publication . Article . Other literature type . 2002

Isolation and characterization of polymorphic microsatellites in olive (Olea europaea L.) and their transferability to other genera in the Oleaceae

Kenneth R. Tobutt; C. M. James; R. De La Rosa;
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  • Published: 01 Sep 2002 Journal: Molecular Ecology Notes, volume 2, pages 265-267 (issn: 1471-8278, eissn: 1471-8286, Copyright policy)
  • Publisher: Wiley
Abstract
Seven polymorphic microsatellites were developed in olive. Six of them came from a genomic library enriched for GA and CA repeat sequences. They showed single locus polymorphism in a set of 23 olive cultivars (from six to nine alleles per locus). Three different pairs of loci were sufficient to discriminate all cultivars. The other polymorphic primer pair was designed from a published sequence for olive lupeol sgutase and revealed just two alleles. The seven primer pairs were tested on two accessions of five other species of the Oleaceae and three, EMO2, EMO13 and EMO90, revealed polymorphism in two, four and three species, respectively.
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free text keywords: General Biochemistry, Genetics and Molecular Biology, Ecology, Biochemistry, Oleaceae, biology.organism_classification, biology, Botany, Genetics, Locus (genetics), Olea, Allele, Genus, Polymorphism (computer science), Genomic library, Microsatellite
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