publication . Other literature type . Article . 2014

Site-specific genetic engineering of the Anopheles gambiae Y chromosome

Bernardini, F; Galizi, R; Menichelli, M; Papathanos, P; Dritsou, V; Marois, Eric; Crisanti, A; Windbichler, N;
  • Published: 12 May 2014
  • Publisher: Proceedings of the National Academy of Sciences
Abstract
Despite its function in sex determination and its role in driving genome evolution, the Y chromosome remains poorly understood in most species. Y chromosomes are gene-poor, repeat-rich and largely heterochromatic and therefore represent a difficult target for genetic engineering. The Y chromosome of the human malaria vector Anopheles gambiae appears to be involved in sex determination although very little is known about both its structure and function. Here, we characterize a transgenic strain of this mosquito species, obtained by transposon-mediated integration of a transgene construct onto the Y chromosome. Using meganuclease-induced homologous repair we intro...
Subjects
free text keywords: [SDV.IMM]Life Sciences [q-bio]/Immunology, Biological Sciences, Q1, QH426, Multidisciplinary, Homologous chromosome, Genetics, Anopheles gambiae, biology.organism_classification, biology, Genome evolution, Transgene, Y chromosome, Heterochromatin, Chromosome, Anopheles
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