publication . Article . Other literature type . 2019

Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains.

Michael Wilson; Panagis Filippakopoulos;
Open Access
  • Published: 01 Feb 2019 Journal: Molecular Cell, volume 73, pages 621-63,800,000,000,000,000,000 (issn: 1097-2765, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Highlights • Treatment with JQ1 induces an extensive BET proteins interactome rewiring • Structural and biophysical studies expand the target space for BET bromodomains • Two distinct short linear motifs mediate BET ET domain interactions • BRD3 negatively regulates proliferation through Pol I and II mechanisms
Subjects
free text keywords: Clinic for Gynecology, 610 Medicine & health, Cell Biology, Molecular Biology, Article, BET, bromodomain, proteomic network, JQ1, rewiring, rRNA, nucleolus, KacY, AP-MS, protein crystallography
Funded by
NSERC
Project
  • Funder: Natural Sciences and Engineering Research Council of Canada (NSERC)
,
RCUK| ZMYND8-regulation of MITF as potential therapeutic target in melanoma
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: MR/N010051/1
  • Funding stream: MRC
,
EC| ULTRA-DD
Project
ULTRA-DD
Unrestricted Leveraging of Targets for Research Advancement and Drug Discovery
  • Funder: European Commission (EC)
  • Project Code: 115766
  • Funding stream: FP7 | SP1 | SP1-JTI
,
WT| Structural role of BET bromodomains in transcription.
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 095751
  • Funding stream: Genetics, Genomics and Population Research
,
CIHR
Project
  • Funder: Canadian Institutes of Health Research (CIHR)
75 references, page 1 of 5

Anders, L., Guenther, M.G., Qi, J., Fan, Z.P., Marineau, J.J., Rahl, P.B., Lovén, J., Sigova, A.A., Smith, W.B., Lee, T.I.. Genome-wide localization of small molecules. Nat. Biotechnol.. 2014; 32: 92-96 [OpenAIRE] [PubMed]

Aydin, I., Schelhaas, M.. Viral genome tethering to host cell chromatin: cause and consequences. Traffic. 2016; 17: 327-340 [OpenAIRE] [PubMed]

Basham, M., Filik, J., Wharmby, M.T., Chang, P.C., El Kassaby, B., Gerring, M., Aishima, J., Levik, K., Pulford, B.C., Sikharulidze, I.. Data Analysis WorkbeNch (DAWN). J. Synchrotron Radiat.. 2015; 22: 853-858 [OpenAIRE] [PubMed]

Beavis, R.C.. Using the global proteome machine for protein identification. Methods Mol. Biol.. 2006; 328: 217-228 [OpenAIRE] [PubMed]

Bernstein, B.E., Meissner, A., Lander, E.S.. The mammalian epigenome. Cell. 2007; 128: 669-681 [OpenAIRE] [PubMed]

Bhagwat, A.S., Roe, J.S., Mok, B.Y.L., Hohmann, A.F., Shi, J., Vakoc, C.R.. BET Bromodomain inhibition releases the mediator complex from select cis-regulatory elements. Cell Rep.. 2016; 15: 519-530 [OpenAIRE] [PubMed]

Bradner, J.E., Hnisz, D., Young, R.A.. Transcriptional addiction in cancer. Cell. 2017; 168: 629-643 [OpenAIRE] [PubMed]

Calo, E., Gu, B., Bowen, M.E., Aryan, F., Zalc, A., Liang, J., Flynn, R.A., Swigut, T., Chang, H.Y., Attardi, L.D., Wysocka, J.. Tissue-selective effects of nucleolar stress and rDNA damage in developmental disorders. Nature. 2018; 554: 112-117 [OpenAIRE] [PubMed]

Choi, H., Larsen, B., Lin, Z.Y., Breitkreutz, A., Mellacheruvu, D., Fermin, D., Qin, Z.S., Tyers, M., Gingras, A.C., Nesvizhskii, A.I.. SAINT: probabilistic scoring of affinity purification-mass spectrometry data. Nat. Methods. 2011; 8: 70-73 [OpenAIRE] [PubMed]

Crowe, B.L., Larue, R.C., Yuan, C., Hess, S., Kvaratskhelia, M., Foster, M.P.. Structure of the Brd4 ET domain bound to a C-terminal motif from γ-retroviral integrases reveals a conserved mechanism of interaction. Proc. Natl. Acad. Sci. USA. 2016; 113: 2086-2091 [OpenAIRE] [PubMed]

Dawson, M.A., Prinjha, R.K., Dittmann, A., Giotopoulos, G., Bantscheff, M., Chan, W.I., Robson, S.C., Chung, C.W., Hopf, C., Savitski, M.M.. Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia. Nature. 2011; 478: 529-533 [OpenAIRE] [PubMed]

Delmore, J.E., Issa, G.C., Lemieux, M.E., Rahl, P.B., Shi, J., Jacobs, H.M., Kastritis, E., Gilpatrick, T., Paranal, R.M., Qi, J.. BET bromodomain inhibition as a therapeutic strategy to target c-Myc. Cell. 2011; 146: 904-917 [OpenAIRE] [PubMed]

Dhalluin, C., Carlson, J.E., Zeng, L., He, C., Aggarwal, A.K., Zhou, M.M.. Structure and ligand of a histone acetyltransferase bromodomain. Nature. 1999; 399: 491-496 [OpenAIRE] [PubMed]

Dooley, K.E., Warburton, A., McBride, A.A.. Tandemly integrated HPV16 can form a Brd4-dependent super-enhancer-like element that drives transcription of viral oncogenes. MBio. 2016; 7

Doroshow, D.B., Eder, J.P., LoRusso, P.M.. BET inhibitors: a novel epigenetic approach. Ann. Oncol.. 2017; 28: 1776-1787 [OpenAIRE] [PubMed]

75 references, page 1 of 5
Abstract
Highlights • Treatment with JQ1 induces an extensive BET proteins interactome rewiring • Structural and biophysical studies expand the target space for BET bromodomains • Two distinct short linear motifs mediate BET ET domain interactions • BRD3 negatively regulates proliferation through Pol I and II mechanisms
Subjects
free text keywords: Clinic for Gynecology, 610 Medicine & health, Cell Biology, Molecular Biology, Article, BET, bromodomain, proteomic network, JQ1, rewiring, rRNA, nucleolus, KacY, AP-MS, protein crystallography
Funded by
NSERC
Project
  • Funder: Natural Sciences and Engineering Research Council of Canada (NSERC)
,
RCUK| ZMYND8-regulation of MITF as potential therapeutic target in melanoma
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: MR/N010051/1
  • Funding stream: MRC
,
EC| ULTRA-DD
Project
ULTRA-DD
Unrestricted Leveraging of Targets for Research Advancement and Drug Discovery
  • Funder: European Commission (EC)
  • Project Code: 115766
  • Funding stream: FP7 | SP1 | SP1-JTI
,
WT| Structural role of BET bromodomains in transcription.
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 095751
  • Funding stream: Genetics, Genomics and Population Research
,
CIHR
Project
  • Funder: Canadian Institutes of Health Research (CIHR)
75 references, page 1 of 5

Anders, L., Guenther, M.G., Qi, J., Fan, Z.P., Marineau, J.J., Rahl, P.B., Lovén, J., Sigova, A.A., Smith, W.B., Lee, T.I.. Genome-wide localization of small molecules. Nat. Biotechnol.. 2014; 32: 92-96 [OpenAIRE] [PubMed]

Aydin, I., Schelhaas, M.. Viral genome tethering to host cell chromatin: cause and consequences. Traffic. 2016; 17: 327-340 [OpenAIRE] [PubMed]

Basham, M., Filik, J., Wharmby, M.T., Chang, P.C., El Kassaby, B., Gerring, M., Aishima, J., Levik, K., Pulford, B.C., Sikharulidze, I.. Data Analysis WorkbeNch (DAWN). J. Synchrotron Radiat.. 2015; 22: 853-858 [OpenAIRE] [PubMed]

Beavis, R.C.. Using the global proteome machine for protein identification. Methods Mol. Biol.. 2006; 328: 217-228 [OpenAIRE] [PubMed]

Bernstein, B.E., Meissner, A., Lander, E.S.. The mammalian epigenome. Cell. 2007; 128: 669-681 [OpenAIRE] [PubMed]

Bhagwat, A.S., Roe, J.S., Mok, B.Y.L., Hohmann, A.F., Shi, J., Vakoc, C.R.. BET Bromodomain inhibition releases the mediator complex from select cis-regulatory elements. Cell Rep.. 2016; 15: 519-530 [OpenAIRE] [PubMed]

Bradner, J.E., Hnisz, D., Young, R.A.. Transcriptional addiction in cancer. Cell. 2017; 168: 629-643 [OpenAIRE] [PubMed]

Calo, E., Gu, B., Bowen, M.E., Aryan, F., Zalc, A., Liang, J., Flynn, R.A., Swigut, T., Chang, H.Y., Attardi, L.D., Wysocka, J.. Tissue-selective effects of nucleolar stress and rDNA damage in developmental disorders. Nature. 2018; 554: 112-117 [OpenAIRE] [PubMed]

Choi, H., Larsen, B., Lin, Z.Y., Breitkreutz, A., Mellacheruvu, D., Fermin, D., Qin, Z.S., Tyers, M., Gingras, A.C., Nesvizhskii, A.I.. SAINT: probabilistic scoring of affinity purification-mass spectrometry data. Nat. Methods. 2011; 8: 70-73 [OpenAIRE] [PubMed]

Crowe, B.L., Larue, R.C., Yuan, C., Hess, S., Kvaratskhelia, M., Foster, M.P.. Structure of the Brd4 ET domain bound to a C-terminal motif from γ-retroviral integrases reveals a conserved mechanism of interaction. Proc. Natl. Acad. Sci. USA. 2016; 113: 2086-2091 [OpenAIRE] [PubMed]

Dawson, M.A., Prinjha, R.K., Dittmann, A., Giotopoulos, G., Bantscheff, M., Chan, W.I., Robson, S.C., Chung, C.W., Hopf, C., Savitski, M.M.. Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia. Nature. 2011; 478: 529-533 [OpenAIRE] [PubMed]

Delmore, J.E., Issa, G.C., Lemieux, M.E., Rahl, P.B., Shi, J., Jacobs, H.M., Kastritis, E., Gilpatrick, T., Paranal, R.M., Qi, J.. BET bromodomain inhibition as a therapeutic strategy to target c-Myc. Cell. 2011; 146: 904-917 [OpenAIRE] [PubMed]

Dhalluin, C., Carlson, J.E., Zeng, L., He, C., Aggarwal, A.K., Zhou, M.M.. Structure and ligand of a histone acetyltransferase bromodomain. Nature. 1999; 399: 491-496 [OpenAIRE] [PubMed]

Dooley, K.E., Warburton, A., McBride, A.A.. Tandemly integrated HPV16 can form a Brd4-dependent super-enhancer-like element that drives transcription of viral oncogenes. MBio. 2016; 7

Doroshow, D.B., Eder, J.P., LoRusso, P.M.. BET inhibitors: a novel epigenetic approach. Ann. Oncol.. 2017; 28: 1776-1787 [OpenAIRE] [PubMed]

75 references, page 1 of 5
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