publication . Article . Other literature type . 2013

Global Patterns of Tissue-Specific Alternative Polyadenylation in Drosophila

Pedro Miura; Justen Andrews; Peter Cherbas; Peter Smibert; Dayu Zhang; Thomas C. Kaufman; Joe Carlson; Gemma E. May; James B. Brown; Eric C. Lai; ...
Open Access English
  • Published: 01 Mar 2013 Journal: Cell Reports, issue 3, pages 277-289 (issn: 22111247, Copyright policy)
  • Publisher: The Authors. Published by Elsevier Inc.
Abstract
SummaryWe analyzed the usage and consequences of alternative cleavage and polyadenylation (APA) in Drosophila melanogaster by using >1 billion reads of stranded mRNA-seq across a variety of dissected tissues. Beyond demonstrating that a majority of fly transcripts are subject to APA, we observed broad trends for 3′ untranslated region (UTR) shortening in the testis and lengthening in the central nervous system (CNS); the latter included hundreds of unannotated extensions ranging up to 18 kb. Extensive northern analyses validated the accumulation of full-length neural extended transcripts, and in situ hybridization indicated their spatial restriction to the CNS. ...
Subjects
free text keywords: Article, General Biochemistry, Genetics and Molecular Biology, Biology (General), QH301-705.5, Polyadenylation, Three prime untranslated region, RNA, RNA-binding protein, Drosophila melanogaster, biology.organism_classification, biology, Genetics, Gene, Untranslated region, Gene expression
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