publication . Article . 2018

A synthetic homing endonuclease-based gene drive system in the human malaria mosquito

Windbichler Nikolai; Menichelli Miriam; Papathanos Philippos Aris; Thyme Summer B.; Li Hui; Ulge Umut Y.; Hovde Blake T.; Baker David; Monnat Raymond J.; Burt Austin; ...
Open Access English
  • Published: 13 Nov 2018
  • Publisher: NATURE PUBLISHING GROUP
  • Country: United Kingdom
Abstract
Genetic approaches to manipulating or eradicating disease vectors have been proposed as alternatives to malaria eradication. The success of this approach depends on efficient spread of a genetic modification in field populations. Windbichler et al. show that a synthetic genetic element consisting of mosquito regulatory elements and the homing endonuclease gene I-SceI can spread from a small number of individual Anopheles gambiae mosquitoes into large receptive populations in just a few generations. This is the first demonstration of a synthetic gene drive system in the main human malaria vector — and a similar approach should be applicable to many other pest spe...
Subjects
Medical Subject Headings: parasitic diseases
free text keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, SPECIFICITY, POPULATIONS, DROSOPHILA, BINDING, TRANSMISSION, DERIVATIVES, GENERATION, CONVERSION, PARASITE, SEQUENCE, Animals, Animals, Genetically Modified, Anopheles gambiae, Deoxyribonucleases, Type II Site-Specific, Female, Genes, Reporter, Genetic Engineering, Genotype, Insect Vectors, Male, Molecular Sequence Data, Mosquito Control, Saccharomyces cerevisiae Proteins, MD Multidisciplinary, General Science & Technology, Article, Homing endonuclease, biology.protein, biology, Gene conversion, Vector (epidemiology), Gene drive, Gene, Computational biology, biology.organism_classification, Genetics, Transgene
30 references, page 1 of 2

Curtis, CF. Possible use of translocations to fix desirable genes in insect pest populations. Nature. 1968; 218: 368-369 [PubMed]

Hamilton, WD. Extraordinary sex ratios. A sex-ratio theory for sex linkage and inbreeding has new implications in cytogenetics and entomology. Science. 1967; 156: 477-488 [PubMed]

Alphey, L. Malaria control with genetically manipulated insect vectors. Science. 2002; 298: 119-121 [OpenAIRE] [PubMed]

Corby-Harris, V. Activation of Akt Signaling Reduces the Prevalence and Intensity of Malaria Parasite Infection and Lifespan in Anopheles stephensi Mosquitoes. PLoS Pathog. 2010; 6: e1001003 [OpenAIRE] [PubMed]

Ito, J, Ghosh, A, Moreira, LA, Wimmer, EA, Jacobs-Lorena, M. Transgenic anopheline mosquitoes impaired in transmission of a malaria parasite. Nature. 2002; 417: 452-455 [OpenAIRE] [PubMed] [DOI]

Moreira, LA. Bee venom phospholipase inhibits malaria parasite development in transgenic mosquitoes. J Biol Chem. 2002; 277: 40839-40843 [OpenAIRE] [PubMed] [DOI]

Li, F, Patra, KP, Vinetz, JM. An anti-Chitinase malaria transmission-blocking single-chain antibody as an effector molecule for creating a Plasmodium falciparum-refractory mosquito. J Infect Dis. 2005; 192: 878-887 [OpenAIRE] [PubMed] [DOI]

Sinkins, SP, Gould, F. Gene drive systems for insect disease vectors. Nat Rev Genet. 2006; 7: 427-435 [OpenAIRE] [PubMed]

Burt, A. Site-specific selfish genes as tools for the control and genetic engineering of natural populations. Proc Biol Sci. 2003; 270: 921-928 [OpenAIRE] [PubMed]

Catteruccia, F, Benton, JP, Crisanti, A. An Anopheles transgenic sexing strain for vector control. Nat Biotechnol. 2005; 23: 1414-1417 [OpenAIRE] [PubMed]

Jacquier, A, Dujon, B. An intron-encoded protein is active in a gene conversion process that spreads an intron into a mitochondrial gene. Cell. 1985; 41: 383-394 [PubMed]

Bellaiche, Y, Mogila, V, Perrimon, N. I-SceI endonuclease, a new tool for studying DNA double-strand break repair mechanisms in Drosophila. Genetics. 1999; 152: 1037-1044 [OpenAIRE] [PubMed]

Windbichler, N. Homing endonuclease mediated gene targeting in Anopheles gambiae cells and embryos. Nucleic Acids Res. 2007; 35: 5922-5933 [OpenAIRE] [PubMed]

Stoddard, BL. Homing endonuclease structure and function. Q Rev Biophys. 2005; 38: 49-95 [PubMed]

Goddard, MR, Greig, D, Burt, A. Outcrossed sex allows a selfish gene to invade yeast populations. Proc Biol Sci. 2001; 268: 2537-2542 [OpenAIRE] [PubMed] [DOI]

30 references, page 1 of 2
Abstract
Genetic approaches to manipulating or eradicating disease vectors have been proposed as alternatives to malaria eradication. The success of this approach depends on efficient spread of a genetic modification in field populations. Windbichler et al. show that a synthetic genetic element consisting of mosquito regulatory elements and the homing endonuclease gene I-SceI can spread from a small number of individual Anopheles gambiae mosquitoes into large receptive populations in just a few generations. This is the first demonstration of a synthetic gene drive system in the main human malaria vector — and a similar approach should be applicable to many other pest spe...
Subjects
Medical Subject Headings: parasitic diseases
free text keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, SPECIFICITY, POPULATIONS, DROSOPHILA, BINDING, TRANSMISSION, DERIVATIVES, GENERATION, CONVERSION, PARASITE, SEQUENCE, Animals, Animals, Genetically Modified, Anopheles gambiae, Deoxyribonucleases, Type II Site-Specific, Female, Genes, Reporter, Genetic Engineering, Genotype, Insect Vectors, Male, Molecular Sequence Data, Mosquito Control, Saccharomyces cerevisiae Proteins, MD Multidisciplinary, General Science & Technology, Article, Homing endonuclease, biology.protein, biology, Gene conversion, Vector (epidemiology), Gene drive, Gene, Computational biology, biology.organism_classification, Genetics, Transgene
30 references, page 1 of 2

Curtis, CF. Possible use of translocations to fix desirable genes in insect pest populations. Nature. 1968; 218: 368-369 [PubMed]

Hamilton, WD. Extraordinary sex ratios. A sex-ratio theory for sex linkage and inbreeding has new implications in cytogenetics and entomology. Science. 1967; 156: 477-488 [PubMed]

Alphey, L. Malaria control with genetically manipulated insect vectors. Science. 2002; 298: 119-121 [OpenAIRE] [PubMed]

Corby-Harris, V. Activation of Akt Signaling Reduces the Prevalence and Intensity of Malaria Parasite Infection and Lifespan in Anopheles stephensi Mosquitoes. PLoS Pathog. 2010; 6: e1001003 [OpenAIRE] [PubMed]

Ito, J, Ghosh, A, Moreira, LA, Wimmer, EA, Jacobs-Lorena, M. Transgenic anopheline mosquitoes impaired in transmission of a malaria parasite. Nature. 2002; 417: 452-455 [OpenAIRE] [PubMed] [DOI]

Moreira, LA. Bee venom phospholipase inhibits malaria parasite development in transgenic mosquitoes. J Biol Chem. 2002; 277: 40839-40843 [OpenAIRE] [PubMed] [DOI]

Li, F, Patra, KP, Vinetz, JM. An anti-Chitinase malaria transmission-blocking single-chain antibody as an effector molecule for creating a Plasmodium falciparum-refractory mosquito. J Infect Dis. 2005; 192: 878-887 [OpenAIRE] [PubMed] [DOI]

Sinkins, SP, Gould, F. Gene drive systems for insect disease vectors. Nat Rev Genet. 2006; 7: 427-435 [OpenAIRE] [PubMed]

Burt, A. Site-specific selfish genes as tools for the control and genetic engineering of natural populations. Proc Biol Sci. 2003; 270: 921-928 [OpenAIRE] [PubMed]

Catteruccia, F, Benton, JP, Crisanti, A. An Anopheles transgenic sexing strain for vector control. Nat Biotechnol. 2005; 23: 1414-1417 [OpenAIRE] [PubMed]

Jacquier, A, Dujon, B. An intron-encoded protein is active in a gene conversion process that spreads an intron into a mitochondrial gene. Cell. 1985; 41: 383-394 [PubMed]

Bellaiche, Y, Mogila, V, Perrimon, N. I-SceI endonuclease, a new tool for studying DNA double-strand break repair mechanisms in Drosophila. Genetics. 1999; 152: 1037-1044 [OpenAIRE] [PubMed]

Windbichler, N. Homing endonuclease mediated gene targeting in Anopheles gambiae cells and embryos. Nucleic Acids Res. 2007; 35: 5922-5933 [OpenAIRE] [PubMed]

Stoddard, BL. Homing endonuclease structure and function. Q Rev Biophys. 2005; 38: 49-95 [PubMed]

Goddard, MR, Greig, D, Burt, A. Outcrossed sex allows a selfish gene to invade yeast populations. Proc Biol Sci. 2001; 268: 2537-2542 [OpenAIRE] [PubMed] [DOI]

30 references, page 1 of 2
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