publication . Article . 2017

The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy

Hesketh, EL; Meschcheriakova, Y; Thompson, RF; Lomonossoff, GP; Ranson, NA;
Open Access
  • Published: 03 Apr 2017 Journal: Scientific Reports, volume 7 (eissn: 2045-2322, Copyright policy)
  • Publisher: Springer Nature
  • Country: United Kingdom
Abstract
Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the develo...
Subjects
Medical Subject Headings: viruses
free text keywords: Article
Funded by
RCUK| Untangling the processes of replication and encapsidation in Picornavirales
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: BB/L020955/1
  • Funding stream: BBSRC
,
WT| The Structural Basis of Biological Mechanisms
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 096685
  • Funding stream: Cellular and Molecular Neuroscience
,
WT| New infrastructure for EM studies at the University of Leeds.
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 090932
  • Funding stream: Cellular and Molecular Neuroscience
,
WT| Structural Molecular Biology Infrastructure for the Astbury Centre at Leeds.
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 094232
  • Funding stream: Cellular and Molecular Neuroscience

Additional Information Supplementary information accompanies this paper at doi:10.1038/s41598-017-00533-w

Abstract
Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the develo...
Subjects
Medical Subject Headings: viruses
free text keywords: Article
Funded by
RCUK| Untangling the processes of replication and encapsidation in Picornavirales
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: BB/L020955/1
  • Funding stream: BBSRC
,
WT| The Structural Basis of Biological Mechanisms
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 096685
  • Funding stream: Cellular and Molecular Neuroscience
,
WT| New infrastructure for EM studies at the University of Leeds.
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 090932
  • Funding stream: Cellular and Molecular Neuroscience
,
WT| Structural Molecular Biology Infrastructure for the Astbury Centre at Leeds.
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 094232
  • Funding stream: Cellular and Molecular Neuroscience

Additional Information Supplementary information accompanies this paper at doi:10.1038/s41598-017-00533-w

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