publication . Article . Other literature type . 2019

Differences in protein structural regions that impact functional specificity in GT2 family β-glucan synthases

Oehme, Daniel P.; Shafee, Thomas; Downton, Matthew T.; Bacic, Antony; Doblin, Monika S.;
Open Access English
  • Published: 30 Oct 2019 Journal: PLoS ONE (issn: 1932-6203, Copyright policy)
  • Publisher: Public Library of Science (PLoS)
Abstract
Most cell wall and secreted β-glucans are synthesised by the CAZy Glycosyltransferase 2 family (www.cazy.org), with different members catalysing the formation of (1,4)-β-, (1,3)-β-, or both (1,4)- and (1,3)-β-glucosidic linkages. Given the distinct physicochemical properties of each of the resultant β-glucans (cellulose, curdlan, and mixed linkage glucan, respectively) are crucial to their biological and biotechnological functions, there is a desire to understand the molecular evolution of synthesis and how linkage specificity is determined. With structural studies hamstrung by the instability of these proteins to solubilisation, we have utilised in silico techn...
Subjects
free text keywords: Medicine, R, Science, Q, Research Article, Research and Analysis Methods, Database and Informatics Methods, Bioinformatics, Sequence Analysis, Sequence Motif Analysis, Sequence Alignment, Physical Sciences, Physics, Condensed Matter Physics, Solid State Physics, Crystallography, Crystal Structure, Biology and Life Sciences, Biochemistry, Glycobiology, Polysaccharides, Glucans, Computational Techniques, Split-Decomposition Method, Multiple Alignment Calculation, Biochemical Simulations, Computational Biology, Chemistry, Chemical Compounds, Organic Compounds, Cellulose, Organic Chemistry, Biophysics, Ion Channels, Transient Receptor Potential Channels, Physiology, Electrophysiology, Medicine and Health Sciences, Neurophysiology, Neuroscience, Proteins, General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Curdlan, chemistry.chemical_compound, In silico, CAZy, Cell wall, Protein structure, Mixed-linkage glucan, Homology modeling
Related Organizations
Download fromView all 4 versions
PLoS ONE
Article . 2019
PLoS ONE
Article . 2019
Provider: Crossref
PLoS ONE
Article
Provider: UnpayWall
90 references, page 1 of 6

1 Pérez-Mendoza D, Rodríguez-Carvajal MÁ, Romero-Jiménez L, Farias GDA, Lloret J, Gallegos MT, et al Novel mixed-linkage β-glucan activated by c-di-GMP in Sinorhizobium meliloti. Proc Natl Acad Sci 2015;112:E757–65. 10.1073/pnas.1421748112 25650430 [OpenAIRE] [PubMed] [DOI]

2 Burton RA, Wilson SM, Hrmova M, Harvey AJ, Shirley NJ, Medhurst A, et al Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-β-D-glucans. Science 2006;311:1940–2. 10.1126/science.1122975 16574868 [OpenAIRE] [PubMed] [DOI]

3 Doblin MS, Pettolino FA, Wilson SM, Campbell R, Burton RA, Fincher GB, et al A barley cellulose synthase-like CSLH gene in transgenic Arabidopsis 2009;106:5996–6001. 10.1073/pnas.0902019106 19321749 [OpenAIRE] [PubMed] [DOI]

4 Fincher GB. Exploring the evolution of (1,3;1,4)-β-D-glucans in plant cell walls: comparative genomics can help!Curr Opin Plant Biol 2009;12:140–7. 10.1016/j.pbi.2009.01.002 19168383 [OpenAIRE] [PubMed] [DOI]

5 Little A, Schwerdt JG, Shirley NJ, Khor SF, Neumann K, O’Donovan LA, et al Revised Phylogeny of the Cellulose Synthase Gene Superfamily: Insights into Cell Wall Evolution. Plant Physiol 2018;177:1124–41. 10.1104/pp.17.01718 29780036 [OpenAIRE] [PubMed] [DOI]

6 Collins HM, Burton RA, Topping DL, Liao M-L, Bacic A, Fincher GB. Variability in fine structures of noncellulosic cell wall polysaccharides from cereal grains: potential importance in human health and nutrition. Ce real Chem 2010;87:272–82. 10.1094/CCHEM-87-4-0272 [DOI]

7 Johnson KL, Gidley MJ, Bacic A, Doblin MS. Cell wall biomechanics: a tractable challenge in manipulating plant cell walls ‘fit for purpose’!Curr Opin Biotechnol 2018;49:163–71. 10.1016/j.copbio.2017.08.013 28915438 [OpenAIRE] [PubMed] [DOI]

8 Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B. The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res 2014;42:490–5. 10.1093/nar/gkt1178 24270786 [OpenAIRE] [PubMed] [DOI]

9 Römling U, Galperin MY. Bacterial cellulose biosynthesis: Diversity of operons, subunits, products, and functions. Trends Microbiol 2015;23:545–57. 10.1016/j.tim.2015.05.005 26077867 [OpenAIRE] [PubMed] [DOI]

10 Grenville-Briggs LJ, Anderson VL, Fugelstad J, Avrova AO, Bouzenzana J, Williams A, et al Cellulose Synthesis in Phytophthora infestans Is Required for Normal Appressorium Formation and Successful Infection of Potato. Plant Cell 2008;20:720–38. 10.1105/tpc.107.052043 18349153 [OpenAIRE] [PubMed] [DOI]

11 McFarlane HE, Döring A, Persson S. The cell biology of cellulose synthesis. Annu Rev Plant Biol 2014;65:69–94. 10.1146/annurev-arplant-050213-040240 24579997 [OpenAIRE] [PubMed] [DOI]

12 Stasinopoulos SJ, Fisher PR, Stone B a., Stanisich V a. Detection of two loci involved in (1->3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene. Glycobiology 1999;9:31–41. 10.1093/glycob/9.1.31 9884404 [OpenAIRE] [PubMed] [DOI]

13 Sethaphong L, Haigler CH, Kubicki JD, Zimmer J, Bonetta D, DeBolt S, et al Tertiary model of a plant cellulose synthase. Proc Natl Acad Sci U S A 2013;110:7512–7. 10.1073/pnas.1301027110 23592721 [OpenAIRE] [PubMed] [DOI]

14 Douglas CM, Foor F, Marrinan JA, Morin N, Nielsen JB, Dahl AM, et al The Saccharomyces cerevisiae FKS1 (ETG1) gene encodes an integral membrane protein which is a subunit of 1,3-beta-D-glucan synthase. Proc Natl Acad Sci 1994;91:12907–11. 10.1073/pnas.91.26.12907 7528927 [OpenAIRE] [PubMed] [DOI]

15 Ellinger D, Voigt CA. Callose biosynthesis in arabidopsis with a focus on pathogen response: What we have learned within the last decade. Ann Bot 2014;114:1349–58. 10.1093/aob/mcu120 24984713 [OpenAIRE] [PubMed] [DOI]

90 references, page 1 of 6
Abstract
Most cell wall and secreted β-glucans are synthesised by the CAZy Glycosyltransferase 2 family (www.cazy.org), with different members catalysing the formation of (1,4)-β-, (1,3)-β-, or both (1,4)- and (1,3)-β-glucosidic linkages. Given the distinct physicochemical properties of each of the resultant β-glucans (cellulose, curdlan, and mixed linkage glucan, respectively) are crucial to their biological and biotechnological functions, there is a desire to understand the molecular evolution of synthesis and how linkage specificity is determined. With structural studies hamstrung by the instability of these proteins to solubilisation, we have utilised in silico techn...
Subjects
free text keywords: Medicine, R, Science, Q, Research Article, Research and Analysis Methods, Database and Informatics Methods, Bioinformatics, Sequence Analysis, Sequence Motif Analysis, Sequence Alignment, Physical Sciences, Physics, Condensed Matter Physics, Solid State Physics, Crystallography, Crystal Structure, Biology and Life Sciences, Biochemistry, Glycobiology, Polysaccharides, Glucans, Computational Techniques, Split-Decomposition Method, Multiple Alignment Calculation, Biochemical Simulations, Computational Biology, Chemistry, Chemical Compounds, Organic Compounds, Cellulose, Organic Chemistry, Biophysics, Ion Channels, Transient Receptor Potential Channels, Physiology, Electrophysiology, Medicine and Health Sciences, Neurophysiology, Neuroscience, Proteins, General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Curdlan, chemistry.chemical_compound, In silico, CAZy, Cell wall, Protein structure, Mixed-linkage glucan, Homology modeling
Related Organizations
Download fromView all 4 versions
PLoS ONE
Article . 2019
PLoS ONE
Article . 2019
Provider: Crossref
PLoS ONE
Article
Provider: UnpayWall
90 references, page 1 of 6

1 Pérez-Mendoza D, Rodríguez-Carvajal MÁ, Romero-Jiménez L, Farias GDA, Lloret J, Gallegos MT, et al Novel mixed-linkage β-glucan activated by c-di-GMP in Sinorhizobium meliloti. Proc Natl Acad Sci 2015;112:E757–65. 10.1073/pnas.1421748112 25650430 [OpenAIRE] [PubMed] [DOI]

2 Burton RA, Wilson SM, Hrmova M, Harvey AJ, Shirley NJ, Medhurst A, et al Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-β-D-glucans. Science 2006;311:1940–2. 10.1126/science.1122975 16574868 [OpenAIRE] [PubMed] [DOI]

3 Doblin MS, Pettolino FA, Wilson SM, Campbell R, Burton RA, Fincher GB, et al A barley cellulose synthase-like CSLH gene in transgenic Arabidopsis 2009;106:5996–6001. 10.1073/pnas.0902019106 19321749 [OpenAIRE] [PubMed] [DOI]

4 Fincher GB. Exploring the evolution of (1,3;1,4)-β-D-glucans in plant cell walls: comparative genomics can help!Curr Opin Plant Biol 2009;12:140–7. 10.1016/j.pbi.2009.01.002 19168383 [OpenAIRE] [PubMed] [DOI]

5 Little A, Schwerdt JG, Shirley NJ, Khor SF, Neumann K, O’Donovan LA, et al Revised Phylogeny of the Cellulose Synthase Gene Superfamily: Insights into Cell Wall Evolution. Plant Physiol 2018;177:1124–41. 10.1104/pp.17.01718 29780036 [OpenAIRE] [PubMed] [DOI]

6 Collins HM, Burton RA, Topping DL, Liao M-L, Bacic A, Fincher GB. Variability in fine structures of noncellulosic cell wall polysaccharides from cereal grains: potential importance in human health and nutrition. Ce real Chem 2010;87:272–82. 10.1094/CCHEM-87-4-0272 [DOI]

7 Johnson KL, Gidley MJ, Bacic A, Doblin MS. Cell wall biomechanics: a tractable challenge in manipulating plant cell walls ‘fit for purpose’!Curr Opin Biotechnol 2018;49:163–71. 10.1016/j.copbio.2017.08.013 28915438 [OpenAIRE] [PubMed] [DOI]

8 Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B. The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res 2014;42:490–5. 10.1093/nar/gkt1178 24270786 [OpenAIRE] [PubMed] [DOI]

9 Römling U, Galperin MY. Bacterial cellulose biosynthesis: Diversity of operons, subunits, products, and functions. Trends Microbiol 2015;23:545–57. 10.1016/j.tim.2015.05.005 26077867 [OpenAIRE] [PubMed] [DOI]

10 Grenville-Briggs LJ, Anderson VL, Fugelstad J, Avrova AO, Bouzenzana J, Williams A, et al Cellulose Synthesis in Phytophthora infestans Is Required for Normal Appressorium Formation and Successful Infection of Potato. Plant Cell 2008;20:720–38. 10.1105/tpc.107.052043 18349153 [OpenAIRE] [PubMed] [DOI]

11 McFarlane HE, Döring A, Persson S. The cell biology of cellulose synthesis. Annu Rev Plant Biol 2014;65:69–94. 10.1146/annurev-arplant-050213-040240 24579997 [OpenAIRE] [PubMed] [DOI]

12 Stasinopoulos SJ, Fisher PR, Stone B a., Stanisich V a. Detection of two loci involved in (1->3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene. Glycobiology 1999;9:31–41. 10.1093/glycob/9.1.31 9884404 [OpenAIRE] [PubMed] [DOI]

13 Sethaphong L, Haigler CH, Kubicki JD, Zimmer J, Bonetta D, DeBolt S, et al Tertiary model of a plant cellulose synthase. Proc Natl Acad Sci U S A 2013;110:7512–7. 10.1073/pnas.1301027110 23592721 [OpenAIRE] [PubMed] [DOI]

14 Douglas CM, Foor F, Marrinan JA, Morin N, Nielsen JB, Dahl AM, et al The Saccharomyces cerevisiae FKS1 (ETG1) gene encodes an integral membrane protein which is a subunit of 1,3-beta-D-glucan synthase. Proc Natl Acad Sci 1994;91:12907–11. 10.1073/pnas.91.26.12907 7528927 [OpenAIRE] [PubMed] [DOI]

15 Ellinger D, Voigt CA. Callose biosynthesis in arabidopsis with a focus on pathogen response: What we have learned within the last decade. Ann Bot 2014;114:1349–58. 10.1093/aob/mcu120 24984713 [OpenAIRE] [PubMed] [DOI]

90 references, page 1 of 6
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue