publication . Article . Other literature type . 1997

Neuronal nitric oxide synthase alternatively spliced forms: Prominent functional localizations in the brain

Eliasson, M. J. L.; Blackshaw, S.; Schell, M. J.; Snyder, S. H.;
Open Access
  • Published: 01 Apr 1997 Journal: Proceedings of the National Academy of Sciences, volume 94, pages 3,396-3,401 (issn: 0027-8424, eissn: 1091-6490, Copyright policy)
  • Publisher: Proceedings of the National Academy of Sciences
Neuronal nitric-oxide synthase (nNOS) is subject to alternative splicing. In mice with targeted deletions of exon 2 (nNOSΔ/Δ), two alternatively spliced forms, nNOSβ and γ, which lack exon 2, have been described. We have compared localizations of native nNOSα and nNOSβ and γ by in situ hybridization and immunohistochemistry in wild-type and nNOSΔ/Δ mice. To assess nNOS catalytic activity in intact animals we localized citrulline, which is formed stoichiometrically with NO, by immunohistochemistry. nNOSβ is prominent in several brain regions of wild-type animals and shows 2-to 3-fold up-regulation in the cortex and striatum of nNOSΔ/Δ animals. The persistence of ...
free text keywords: Multidisciplinary, Biological Sciences
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