publication . Article . Other literature type . 1984

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Boeke Jef D.; La Croute Francois; Fink Gerald R.;
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  • Published: 01 Nov 1984 Journal: Molecular and General Genetics MGG, volume 197, pages 345-346 (issn: 0026-8925, eissn: 1432-1874, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
Abstract
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3 + gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3 − mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.
Subjects
free text keywords: Genetics, Molecular Biology, Yeast, Biochemistry, URA3, Orotidine-5'-phosphate decarboxylase activity, Mutant, Enzyme, chemistry.chemical_classification, chemistry, Saccharomyces cerevisiae, biology.organism_classification, biology, Orotic acid, medicine.drug, medicine, Orotidine 5'-phosphate decarboxylase
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Abstract
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3 + gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3 − mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.
Subjects
free text keywords: Genetics, Molecular Biology, Yeast, Biochemistry, URA3, Orotidine-5'-phosphate decarboxylase activity, Mutant, Enzyme, chemistry.chemical_classification, chemistry, Saccharomyces cerevisiae, biology.organism_classification, biology, Orotic acid, medicine.drug, medicine, Orotidine 5'-phosphate decarboxylase
Related Organizations
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