publication . Other literature type . Article . 2010

High-Throughput and Multiplexed LC/MS/MRM Method for Targeted Metabolomics

Ru Wei; Guodong Li; Albert B. Seymour;
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  • Published: 01 Jul 2010
  • Publisher: American Chemical Society (ACS)
Abstract
Target-based metabolomics, focused on a subset of metabolites representative of key pathways, is a valuable tool for assessing metabolic changes resulting from genetic mutation, altered gene expression, and protein dysfunction in a given disease state or as a consequence of an environmental perturbation, such as a pharmaceutical. However, simultaneously analyzing hundreds of endogenous metabolites presents a challenge because of their diverse chemical structures and properties. In this study, we report a high-throughput, sensitive, and reproducible method for target-based metabolomics studies. It combines different separation conditions, optimal ionization polar...
Subjects
free text keywords: Analytical Chemistry
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