publication . Other literature type . Article . 2004

Selection of topoisomerase mutations and overexpression of adeB mRNA transcripts during an outbreak of Acinetobacter baumannii

Paul G. Higgins; Hilmar Wisplinghoff; Danuta Stefanik; Harald Seifert;
  • Published: 01 Oct 2004
  • Publisher: Oxford University Press (OUP)
Abstract
Objectives: To investigate the mechanism of ciprofloxacin resistance in isolates of Acinetobacter baumannii during two hospital outbreaks and to determine the expression level of the gene encoding the AdeB efflux pump. Methods: Isolates were previously typed by PFGE and their MICs determined by broth microdilution. The gyrA and parC genes were sequenced and the adeB gene examined by real-time reverse transcription PCR (RT-PCR). Results: Two clonal lineages were responsible for the two hospital outbreaks. In both outbreaks, ciprofloxacin susceptibility was reduced during the course of the outbreak when compared with the index isolates. Mutations in gyrA and parC ...
Subjects
Medical Subject Headings: biochemical phenomena, metabolism, and nutritionbacterial infections and mycosesbacteria
free text keywords: Pharmacology (medical), Pharmacology, Infectious Diseases, Acinetobacter baumannii, biology.organism_classification, biology, Pulsed-field gel electrophoresis, Neisseriaceae, Virology, Microbiology, Gene, Ciprofloxacin, medicine.drug, medicine, Outbreak, Broth microdilution, Genetics, Mutation, medicine.disease_cause
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publication . Other literature type . Article . 2004

Selection of topoisomerase mutations and overexpression of adeB mRNA transcripts during an outbreak of Acinetobacter baumannii

Paul G. Higgins; Hilmar Wisplinghoff; Danuta Stefanik; Harald Seifert;