publication . Article . Other literature type . 2010

a data set of human endogenous protein ubiquitination sites

Yi Shi; Doug W Chan; Sung Yun Jung; Anna Malovannaya; Yi Wang; Jun Qin;
Open Access
  • Published: 24 Oct 2010 Journal: Molecular & Cellular Proteomics, volume 10, page M110.002089 (issn: 1535-9476, eissn: 1535-9484, Copyright policy)
  • Publisher: American Society for Biochemistry & Molecular Biology (ASBMB)
Lysine ubiquitination is an important and versatile protein post-translational modification. Numerous cellular functions are regulated by ubiquitination, suggesting that extensive numbers of proteins, if not all, are modified with ubiquitin at certain times. However, proteome-wide profiling of ubiquitination sites in the mammalian system is technically challenging. We report the design and characterization of an engineered protein affinity reagent for the isolation of ubiquitinated proteins and the identification of ubiquitination sites with mass spectrometry. This recombinant protein consists of four tandem repeats of ubiquitin-associated domain from UBQLN1 fus...
free text keywords: Analytical Chemistry, Biochemistry, Molecular Biology, Ubiquitin-conjugating enzyme, Hsp70, Ubiquitin, biology.protein, biology, Proteome, F-box protein, Lysine, Proteasome, Protein ubiquitination, Special Issue
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