publication . Article . Other literature type . 2010

Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties

Bartosh, Thomas J.; Ylöstalo, Joni H.; Mohammadipoor, Arezoo; Bazhanov, Nikolay; Coble, Katie; Claypool, Kent; Lee, Ryang Hwa; Choi, Hosoon; Prockop, Darwin J.;
Open Access
  • Published: 19 Jul 2010 Journal: Proceedings of the National Academy of Sciences, volume 107, pages 13,724-13,729 (issn: 0027-8424, eissn: 1091-6490, Copyright policy)
  • Publisher: Proceedings of the National Academy of Sciences
Abstract
Previous reports suggested that culture as 3D aggregates or as spheroids can increase the therapeutic potential of the adult stem/progenitor cells referred to as mesenchymal stem cells or multipotent mesenchymal stromal cells (MSCs). Here we used a hanging drop protocol to prepare human MSCs (hMSCs) as spheroids that maximally expressed TNFα stimulated gene/protein 6 (TSG-6), the antiinflammatory protein that was expressed at high levels by hMSCs trapped in the lung after i.v. infusion and that largely explained the beneficial effects of hMSCs in mice with myocardial infarcts. The properties of spheroid hMSCs were found to depend critically on the culture condit...
Subjects
Medical Subject Headings: endocrine systemequipment and supplies
free text keywords: Cell adhesion, Molecular biology, Progenitor cell, Inflammation, medicine.symptom, medicine, Cell biology, Cell aggregation, Tumor necrosis factor alpha, Spheroid, Cell adhesion molecule, Mesenchymal stem cell, Biology, Biological Sciences
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publication . Article . Other literature type . 2010

Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties

Bartosh, Thomas J.; Ylöstalo, Joni H.; Mohammadipoor, Arezoo; Bazhanov, Nikolay; Coble, Katie; Claypool, Kent; Lee, Ryang Hwa; Choi, Hosoon; Prockop, Darwin J.;