publication . Article . Other literature type . 2018

Combinatorial programming of human neuronal progenitors using magnetically-guided stoichiometric mRNA delivery

Azimi, Sayyed M; Sheridan, Steven D; Ghannad-Rezaie, Mostafa; Eimon, Peter M; Yanik, Mehmet Fatih;
Open Access English
  • Published: 01 May 2018
  • Publisher: eLife Sciences Publications, Ltd
Abstract
<jats:p>Identification of optimal transcription factor expression patterns to direct cellular differentiation along a desired pathway presents significant challenges. We demonstrate massively combinatorial screening of temporally-varying mRNA transcription factors to direct differentiation of neural progenitor cells using a dynamically-reconfigurable magnetically-guided spotting technology for localizing mRNA, enabling experiments on millimetre size spots. In addition, we present a time-interleaved delivery method that dramatically reduces fluctuations in the delivered transcription factor copy numbers per cell. We screened combinatorial and temporal delivery of...
Subjects
free text keywords: neuron, stem cell, reprogramming, high-throughput, screening, transcription factors, neuron type, dopaminergic neurons, neuronal differentiation, Human, Institute of Neuroinformatics, 570 Life sciences; biology, Life sciences, General Biochemistry, Genetics and Molecular Biology, General Immunology and Microbiology, General Neuroscience, General Medicine, Tools and Resources, Neuroscience, info:eu-repo/classification/ddc/570, ddc:570
43 references, page 1 of 3

Abeliovich, A, Hammond, R. Midbrain dopamine neuron differentiation: factors and fates. Developmental Biology. 2007; 304: 447-454 [OpenAIRE] [PubMed] [DOI]

Ang, SL. Transcriptional control of midbrain dopaminergic neuron development. Development. 2006; 133: 3499-3506 [OpenAIRE] [PubMed] [DOI]

Angel, M, Yanik, MF. Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins. PLoS One. 2010; 5 [OpenAIRE] [PubMed] [DOI]

Carpenter, AE, Jones, TR, Lamprecht, MR, Clarke, C, Kang, IH, Friman, O, Guertin, DA, Chang, JH, Lindquist, RA, Moffat, J, Golland, P, Sabatini, DM. CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biology. 2006; 7 [OpenAIRE] [PubMed] [DOI]

Engel, M, Do-Ha, D, Muñoz, SS, Ooi, L. Common pitfalls of stem cell differentiation: a guide to improving protocols for neurodegenerative disease models and research. Cellular and Molecular Life Sciences. 2016; 73: 3693-3709 [OpenAIRE] [PubMed] [DOI]

Friling, S, Andersson, E, Thompson, LH, Jönsson, ME, Hebsgaard, JB, Nanou, E, Alekseenko, Z, Marklund, U, Kjellander, S, Volakakis, N, Hovatta, O, El Manira, A, Björklund, A, Perlmann, T, Ericson, J. Efficient production of mesencephalic dopamine neurons by Lmx1a expression in embryonic stem cells. PNAS. 2009; 106: 7613-7618 [OpenAIRE] [PubMed] [DOI]

Ghannad-Rezaie, M. Github. 2018

Hansson, ML, Albert, S, González Somermeyer, L, Peco, R, Mejía-Ramírez, E, Montserrat, N, Izpisua Belmonte, JC. Efficient delivery and functional expression of transfected modified mRNA in human embryonic stem cell-derived retinal pigmented epithelial cells. Journal of Biological Chemistry. 2015; 290: 5661-5672 [OpenAIRE] [PubMed] [DOI]

Hegarty, SV, Sullivan, AM, O'Keeffe, GW. Midbrain dopaminergic neurons: a review of the molecular circuitry that regulates their development. Developmental Biology. 2013; 379: 123-138 [OpenAIRE] [PubMed] [DOI]

Jemielity, J, Fowler, T, Zuberek, J, Stepinski, J, Lewdorowicz, M, Niedzwiecka, A, Stolarski, R, Darzynkiewicz, E, Rhoads, RE. Novel "anti-reverse" cap analogs with superior translational properties. Rna. 2003; 9: 1108-1122 [OpenAIRE] [PubMed] [DOI]

Jiang, Y, Xu, XS, Russell, JE. A nucleolin-binding 3' untranslated region element stabilizes beta-globin mRNA in vivo. Molecular and Cellular Biology. 2006; 26: 2419-2429 [OpenAIRE] [PubMed] [DOI]

Karikó, K, Buckstein, M, Ni, H, Weissman, D. Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA. Immunity. 2005; 23: 165-175 [OpenAIRE] [PubMed] [DOI]

Karikó, K, Muramatsu, H, Keller, JM, Weissman, D. Increased erythropoiesis in mice injected with submicrogram quantities of pseudouridine-containing mRNA encoding erythropoietin. Molecular therapy : the journal of the American Society of Gene Therapy. 2012; 20: 948-953 [OpenAIRE] [PubMed] [DOI]

Karikó, K, Muramatsu, H, Welsh, FA, Ludwig, J, Kato, H, Akira, S, Weissman, D. Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. Molecular therapy : the journal of the American Society of Gene Therapy. 2008; 16: 1833-1840 [OpenAIRE] [PubMed] [DOI]

Karikó, K, Weissman, D. Naturally occurring nucleoside modifications suppress the immunostimulatory activity of RNA: implication for therapeutic RNA development. Current Opinion in Drug Discovery & Development. 2007; 10: 523-532 [PubMed]

43 references, page 1 of 3
Abstract
<jats:p>Identification of optimal transcription factor expression patterns to direct cellular differentiation along a desired pathway presents significant challenges. We demonstrate massively combinatorial screening of temporally-varying mRNA transcription factors to direct differentiation of neural progenitor cells using a dynamically-reconfigurable magnetically-guided spotting technology for localizing mRNA, enabling experiments on millimetre size spots. In addition, we present a time-interleaved delivery method that dramatically reduces fluctuations in the delivered transcription factor copy numbers per cell. We screened combinatorial and temporal delivery of...
Subjects
free text keywords: neuron, stem cell, reprogramming, high-throughput, screening, transcription factors, neuron type, dopaminergic neurons, neuronal differentiation, Human, Institute of Neuroinformatics, 570 Life sciences; biology, Life sciences, General Biochemistry, Genetics and Molecular Biology, General Immunology and Microbiology, General Neuroscience, General Medicine, Tools and Resources, Neuroscience, info:eu-repo/classification/ddc/570, ddc:570
43 references, page 1 of 3

Abeliovich, A, Hammond, R. Midbrain dopamine neuron differentiation: factors and fates. Developmental Biology. 2007; 304: 447-454 [OpenAIRE] [PubMed] [DOI]

Ang, SL. Transcriptional control of midbrain dopaminergic neuron development. Development. 2006; 133: 3499-3506 [OpenAIRE] [PubMed] [DOI]

Angel, M, Yanik, MF. Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins. PLoS One. 2010; 5 [OpenAIRE] [PubMed] [DOI]

Carpenter, AE, Jones, TR, Lamprecht, MR, Clarke, C, Kang, IH, Friman, O, Guertin, DA, Chang, JH, Lindquist, RA, Moffat, J, Golland, P, Sabatini, DM. CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biology. 2006; 7 [OpenAIRE] [PubMed] [DOI]

Engel, M, Do-Ha, D, Muñoz, SS, Ooi, L. Common pitfalls of stem cell differentiation: a guide to improving protocols for neurodegenerative disease models and research. Cellular and Molecular Life Sciences. 2016; 73: 3693-3709 [OpenAIRE] [PubMed] [DOI]

Friling, S, Andersson, E, Thompson, LH, Jönsson, ME, Hebsgaard, JB, Nanou, E, Alekseenko, Z, Marklund, U, Kjellander, S, Volakakis, N, Hovatta, O, El Manira, A, Björklund, A, Perlmann, T, Ericson, J. Efficient production of mesencephalic dopamine neurons by Lmx1a expression in embryonic stem cells. PNAS. 2009; 106: 7613-7618 [OpenAIRE] [PubMed] [DOI]

Ghannad-Rezaie, M. Github. 2018

Hansson, ML, Albert, S, González Somermeyer, L, Peco, R, Mejía-Ramírez, E, Montserrat, N, Izpisua Belmonte, JC. Efficient delivery and functional expression of transfected modified mRNA in human embryonic stem cell-derived retinal pigmented epithelial cells. Journal of Biological Chemistry. 2015; 290: 5661-5672 [OpenAIRE] [PubMed] [DOI]

Hegarty, SV, Sullivan, AM, O'Keeffe, GW. Midbrain dopaminergic neurons: a review of the molecular circuitry that regulates their development. Developmental Biology. 2013; 379: 123-138 [OpenAIRE] [PubMed] [DOI]

Jemielity, J, Fowler, T, Zuberek, J, Stepinski, J, Lewdorowicz, M, Niedzwiecka, A, Stolarski, R, Darzynkiewicz, E, Rhoads, RE. Novel "anti-reverse" cap analogs with superior translational properties. Rna. 2003; 9: 1108-1122 [OpenAIRE] [PubMed] [DOI]

Jiang, Y, Xu, XS, Russell, JE. A nucleolin-binding 3' untranslated region element stabilizes beta-globin mRNA in vivo. Molecular and Cellular Biology. 2006; 26: 2419-2429 [OpenAIRE] [PubMed] [DOI]

Karikó, K, Buckstein, M, Ni, H, Weissman, D. Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA. Immunity. 2005; 23: 165-175 [OpenAIRE] [PubMed] [DOI]

Karikó, K, Muramatsu, H, Keller, JM, Weissman, D. Increased erythropoiesis in mice injected with submicrogram quantities of pseudouridine-containing mRNA encoding erythropoietin. Molecular therapy : the journal of the American Society of Gene Therapy. 2012; 20: 948-953 [OpenAIRE] [PubMed] [DOI]

Karikó, K, Muramatsu, H, Welsh, FA, Ludwig, J, Kato, H, Akira, S, Weissman, D. Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. Molecular therapy : the journal of the American Society of Gene Therapy. 2008; 16: 1833-1840 [OpenAIRE] [PubMed] [DOI]

Karikó, K, Weissman, D. Naturally occurring nucleoside modifications suppress the immunostimulatory activity of RNA: implication for therapeutic RNA development. Current Opinion in Drug Discovery & Development. 2007; 10: 523-532 [PubMed]

43 references, page 1 of 3
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