publication . Other literature type . Article . 2014

Species tree estimation of North American chorus frogs (Hylidae: Pseudacris) with parallel tagged amplicon sequencing.

Lisa N. Barrow; Hannah F. Ralicki; Emily Moriarty Lemmon; Sandra A. Emme;
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  • Published: 01 Jun 2014
  • Publisher: Elsevier BV
Abstract
Abstract The field of phylogenetics is changing rapidly with the application of high-throughput sequencing to non-model organisms. Cost-effective use of this technology for phylogenetic studies, which often include a relatively small portion of the genome but several taxa, requires strategies for genome partitioning and sequencing multiple individuals in parallel. In this study we estimated a multilocus phylogeny for the North American chorus frog genus Pseudacris using anonymous nuclear loci that were recently developed using a reduced representation library approach. We sequenced 27 nuclear loci and three mitochondrial loci for 44 individuals on 1/3 of an Illu...
Subjects
free text keywords: Genetics, Ecology, Evolution, Behavior and Systematics, Molecular Biology, Clade, Biology, Sanger sequencing, symbols.namesake, symbols, Chorus frog, biology.organism_classification, Phylogenetics, Hylidae, Genome, DNA sequencing, Amplicon
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Abstract
Abstract The field of phylogenetics is changing rapidly with the application of high-throughput sequencing to non-model organisms. Cost-effective use of this technology for phylogenetic studies, which often include a relatively small portion of the genome but several taxa, requires strategies for genome partitioning and sequencing multiple individuals in parallel. In this study we estimated a multilocus phylogeny for the North American chorus frog genus Pseudacris using anonymous nuclear loci that were recently developed using a reduced representation library approach. We sequenced 27 nuclear loci and three mitochondrial loci for 44 individuals on 1/3 of an Illu...
Subjects
free text keywords: Genetics, Ecology, Evolution, Behavior and Systematics, Molecular Biology, Clade, Biology, Sanger sequencing, symbols.namesake, symbols, Chorus frog, biology.organism_classification, Phylogenetics, Hylidae, Genome, DNA sequencing, Amplicon
Related Organizations
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