publication . Article . Other literature type . 2017

Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection

Tang, Anna Ah-San; Tiede, Christian; Hughes, David J.; McPherson, Michael J.; Tomlinson, Darren C.;
Open Access English
  • Published: 14 Nov 2017
  • Country: United Kingdom
Abstract
This work was funded by the University of Leeds. Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding proteins. Artificial binding proteins can be used as reagents to study protein-protein interactions, target posttranslational modifications, and distinguish between homologous proteins. They can also be used as research and affinity reagents, for diagnostic purposes, and as therapeutics. However, the ability to identify isoform-specific reagents remains...
Subjects
free text keywords: QH301 Biology, QD Chemistry, RM Therapeutics. Pharmacology, T-NDAS, QH301, QD, RM, Cell Biology, Biochemistry, Molecular Biology
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