publication . Article . 2017

Different developmental histories of beta-cells generate functional and proliferative heterogeneity during islet growth

Sumeet Pal Singh; Nikolay Ninov; Edward Morrissey; Michael Brand; Özge Kayisoglu; Sharan Janjuha;
Open Access
  • Published: 01 Sep 2017 Journal: Nature Communications, volume 8 (eissn: 2041-1723, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
  • Country: Belgium
Abstract
The proliferative and functional heterogeneity among seemingly uniform cells is a universal phenomenon. Identifying the underlying factors requires single-cell analysis of function and proliferation. Here we show that the pancreatic beta-cells in zebrafish exhibit different growth-promoting and functional properties, which in part reflect differences in the time elapsed since birth of the cells. Calcium imaging shows that the beta-cells in the embryonic islet become functional during early zebrafish development. At later stages, younger beta-cells join the islet following differentiation from post-embryonic progenitors. Notably, the older and younger beta-cells ...
Subjects
free text keywords: Sciences bio-médicales et agricoles, Science, Q, Article
Funded by
EC| ZF-BrainReg
Project
ZF-BrainReg
Regeneration of the adult zebrafish brain
  • Funder: European Commission (EC)
  • Project Code: 669811
  • Funding stream: H2020 | ERC | ERC-ADG
70 references, page 1 of 5

Mascré, G. Distinct contribution of stem and progenitor cells to epidermal maintenance. Nature. 2012; 489: 257-262 [OpenAIRE] [PubMed] [DOI]

Gupta, V, Poss, KD. Clonally dominant cardiomyocytes direct heart morphogenesis. Nature. 2012; 484: 479-484 [OpenAIRE] [PubMed] [DOI]

Bergmann, O. Evidence for cardiomyocyte renewal in humans. Science. 2009; 324: 98-102 [OpenAIRE] [PubMed] [DOI]

Dor, Y, Brown, J, Martinez, OI, Melton, Da. Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Nature. 2004; 429: 41-46 [OpenAIRE] [PubMed] [DOI]

Berasain, C, Avila, MA. Regulation of hepatocyte identity and quiescence. Cell. Mol. Life Sci.. 2015; 72: 3831-3851 [OpenAIRE] [PubMed] [DOI]

Liu, Y, Mziaut, H, Ivanova, A, Solimena, M. β-cells at the crossroads: choosing between insulin granule production and proliferation. Diabetes Obes. Metab.. 2009; 11: 54-64 [OpenAIRE] [PubMed] [DOI]

Xin, Y. RNA sequencing of single human islet cells reveals type 2 diabetes genes. Cell Metab.. 2016 [PubMed]

Xin, Y. Use of the fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells. Proc. Natl Acad. Sci. USA. 2016; 113: 3293-3298 [OpenAIRE] [PubMed] [DOI]

Li, J. Single-cell transcriptomes reveal characteristic features of human pancreatic islet cell types. EMBO Rep.. 2016; 17: 178-187 [OpenAIRE] [PubMed] [DOI]

Ackermann, AM, Wang, Z, Schug, J, Naji, A, Kaestner, KH. Integration of ATAC-seq and RNA-seq identifies human alpha cell and beta cell signature genes. Mol. Metab.. 2016; 5: 233-244 [OpenAIRE] [PubMed] [DOI]

Nica, AC. Cell-type, allelic, and genetic signatures in the human pancreatic beta cell transcriptome. Genome Res.. 2013; 23: 1554-1562 [OpenAIRE] [PubMed] [DOI]

Blodgett, DM. Novel observations from next-generation RNA sequencing of highly purified human adult and fetal islet cell subsets. Diabetes. 2015; 64: 3172-3181 [OpenAIRE] [PubMed] [DOI]

Wang, YJ. Single cell transcriptomics of the human endocrine pancreas. Diabetes. 2016; 65: 3028-3038 [OpenAIRE] [PubMed] [DOI]

14.Baron, M. et al. A single-cell transcriptomic map of the human and mouse pancreas reveals inter- and intra-cell population structure. Cell Syst. 3, 346–360.e4 (2016).

Ellenbroek, JH. Topologically heterogeneous beta cell adaptation in response to high-fat diet in mice. PLoS ONE. 2013; 8: e56922 [OpenAIRE] [PubMed] [DOI]

70 references, page 1 of 5
Abstract
The proliferative and functional heterogeneity among seemingly uniform cells is a universal phenomenon. Identifying the underlying factors requires single-cell analysis of function and proliferation. Here we show that the pancreatic beta-cells in zebrafish exhibit different growth-promoting and functional properties, which in part reflect differences in the time elapsed since birth of the cells. Calcium imaging shows that the beta-cells in the embryonic islet become functional during early zebrafish development. At later stages, younger beta-cells join the islet following differentiation from post-embryonic progenitors. Notably, the older and younger beta-cells ...
Subjects
free text keywords: Sciences bio-médicales et agricoles, Science, Q, Article
Funded by
EC| ZF-BrainReg
Project
ZF-BrainReg
Regeneration of the adult zebrafish brain
  • Funder: European Commission (EC)
  • Project Code: 669811
  • Funding stream: H2020 | ERC | ERC-ADG
70 references, page 1 of 5

Mascré, G. Distinct contribution of stem and progenitor cells to epidermal maintenance. Nature. 2012; 489: 257-262 [OpenAIRE] [PubMed] [DOI]

Gupta, V, Poss, KD. Clonally dominant cardiomyocytes direct heart morphogenesis. Nature. 2012; 484: 479-484 [OpenAIRE] [PubMed] [DOI]

Bergmann, O. Evidence for cardiomyocyte renewal in humans. Science. 2009; 324: 98-102 [OpenAIRE] [PubMed] [DOI]

Dor, Y, Brown, J, Martinez, OI, Melton, Da. Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Nature. 2004; 429: 41-46 [OpenAIRE] [PubMed] [DOI]

Berasain, C, Avila, MA. Regulation of hepatocyte identity and quiescence. Cell. Mol. Life Sci.. 2015; 72: 3831-3851 [OpenAIRE] [PubMed] [DOI]

Liu, Y, Mziaut, H, Ivanova, A, Solimena, M. β-cells at the crossroads: choosing between insulin granule production and proliferation. Diabetes Obes. Metab.. 2009; 11: 54-64 [OpenAIRE] [PubMed] [DOI]

Xin, Y. RNA sequencing of single human islet cells reveals type 2 diabetes genes. Cell Metab.. 2016 [PubMed]

Xin, Y. Use of the fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells. Proc. Natl Acad. Sci. USA. 2016; 113: 3293-3298 [OpenAIRE] [PubMed] [DOI]

Li, J. Single-cell transcriptomes reveal characteristic features of human pancreatic islet cell types. EMBO Rep.. 2016; 17: 178-187 [OpenAIRE] [PubMed] [DOI]

Ackermann, AM, Wang, Z, Schug, J, Naji, A, Kaestner, KH. Integration of ATAC-seq and RNA-seq identifies human alpha cell and beta cell signature genes. Mol. Metab.. 2016; 5: 233-244 [OpenAIRE] [PubMed] [DOI]

Nica, AC. Cell-type, allelic, and genetic signatures in the human pancreatic beta cell transcriptome. Genome Res.. 2013; 23: 1554-1562 [OpenAIRE] [PubMed] [DOI]

Blodgett, DM. Novel observations from next-generation RNA sequencing of highly purified human adult and fetal islet cell subsets. Diabetes. 2015; 64: 3172-3181 [OpenAIRE] [PubMed] [DOI]

Wang, YJ. Single cell transcriptomics of the human endocrine pancreas. Diabetes. 2016; 65: 3028-3038 [OpenAIRE] [PubMed] [DOI]

14.Baron, M. et al. A single-cell transcriptomic map of the human and mouse pancreas reveals inter- and intra-cell population structure. Cell Syst. 3, 346–360.e4 (2016).

Ellenbroek, JH. Topologically heterogeneous beta cell adaptation in response to high-fat diet in mice. PLoS ONE. 2013; 8: e56922 [OpenAIRE] [PubMed] [DOI]

70 references, page 1 of 5
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