publication . Article . 2016

RNAi-mediated knockdown of the voltage gated sodium ion channel TcNav causes mortality in Tribolium castaneum.

Erich Yukio Tempel Nakasu; Martin Edwards;
Open Access English
  • Published: 14 Jul 2016 Journal: Scientific Reports, volume 6 (eissn: 2045-2322, Copyright policy)
  • Publisher: Nature Publishing Group
Abstract
The voltage-gated sodium ion channel (VGSC) belongs to the largest superfamily of ion channels. Since VGSCs play key roles in physiological processes they are major targets for effective insecticides. RNA interference (RNAi) is widely used to analyse gene function, but recently, it has shown potential to contribute to novel strategies for selectively controlling agricultural insect pests. The current study evaluates the delivery of dsRNA targeted to the sodium ion channel paralytic A (TcNav) gene in Tribolium castaneum as a viable means of controlling this insect pest. Delivery of TcNav dsRNA caused severe developmental arrest with larval mortalities up to 73% p...
Subjects
Medical Subject Headings: fungi
free text keywords: Article, Multidisciplinary
70 references, page 1 of 5

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Rinkevich F. D., Du Y. & Dong K. Diversity and convergence of sodium channel mutations involved in resistance to pyrethroids. Pesticide Biochemistry and Physiology 106, 93–100 (2013).24019556 [OpenAIRE] [PubMed]

Dong K.. Molecular biology of insect sodium channels and pyrethroid resistance. Insect Biochem Mol Biol 50, 1–17, doi: 10.1016/j.ibmb.2014.03.012 (2014).24704279 [OpenAIRE] [PubMed] [DOI]

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Loughney K., Kreber R. & Ganetzky B. Molecular analysis of the para locus, a sodium channel gene in Drosophila. Cell 58, 1143–1154 (1989).2550145 [OpenAIRE] [PubMed]

Zhou W., Chung I. B., Liu Z. Q., Goldin A. L. & Dong K. A voltage-gated calcium-selective channel encoded by a sodium channel-like gene. Neuron 42, 101–112, doi: 10.1016/s0896-6273(04)00148-5 (2004).15066268 [OpenAIRE] [PubMed] [DOI]

Ramaswami M. & Tanouye M. A. Two sodium-channel genes in Drosophila: implications for channel diversity. Proceedings of the National Academy of Sciences of the United States of America 86, 2079–2082, doi: 10.1073/pnas.86.6.2079 (1989).2538830 [OpenAIRE] [PubMed] [DOI]

Thackeray J. R. & Ganetzky B. Conserved alternative splicing patterns and splicing signals in the Drosophila sodium channel gene para. Genetics 141, 203–214 (1995).8536968 [OpenAIRE] [PubMed]

Hanrahan C. J., Palladino M. J., Ganetzky B. & Reenan R. A. RNA editing of the Drosophila para Na(+) channel transcript. Evolutionary conservation and developmental regulation. Genetics 155, 1149–1160 (2000).10880477 [OpenAIRE] [PubMed]

Dong K.Insect sodium channels and insecticide resistance. Invertebrat e neuroscience: IN 7, 17–30, doi: 10.1007/s10158-006-0036-9 (2007).17206406 [OpenAIRE] [PubMed] [DOI]

70 references, page 1 of 5
Related research
Abstract
The voltage-gated sodium ion channel (VGSC) belongs to the largest superfamily of ion channels. Since VGSCs play key roles in physiological processes they are major targets for effective insecticides. RNA interference (RNAi) is widely used to analyse gene function, but recently, it has shown potential to contribute to novel strategies for selectively controlling agricultural insect pests. The current study evaluates the delivery of dsRNA targeted to the sodium ion channel paralytic A (TcNav) gene in Tribolium castaneum as a viable means of controlling this insect pest. Delivery of TcNav dsRNA caused severe developmental arrest with larval mortalities up to 73% p...
Subjects
Medical Subject Headings: fungi
free text keywords: Article, Multidisciplinary
70 references, page 1 of 5

Nauen R., Stumpf N. & Elbert A. Toxicological and mechanistic studies on neonicotinoid cross resistance in Q-type Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Manag. Sci. 58, 868–875, doi: 10.1002/ps.557 (2002).12233176 [OpenAIRE] [PubMed] [DOI]

Aktar W., Sengupta D. & Chowdhury A. Impact of pesticides use in agriculture: their benefits and hazards. Interdisciplinary toxicology 2, 1–12 (2009).21217838 [OpenAIRE] [PubMed]

Ware G. W. W. D. M.The pesticide book. (Meister Media Worldwide, 2004).

Defra. Food 2030. Department for Environment, Food and Rural Affairs. P84 (2010).

Yu F. H. & Catterall W. A. Overview of the voltage-gated sodium channel family. Genome biology 4, 207 (2003).12620097 [OpenAIRE] [PubMed]

Rinkevich F. D., Du Y. & Dong K. Diversity and convergence of sodium channel mutations involved in resistance to pyrethroids. Pesticide Biochemistry and Physiology 106, 93–100 (2013).24019556 [OpenAIRE] [PubMed]

Dong K.. Molecular biology of insect sodium channels and pyrethroid resistance. Insect Biochem Mol Biol 50, 1–17, doi: 10.1016/j.ibmb.2014.03.012 (2014).24704279 [OpenAIRE] [PubMed] [DOI]

Catterall W. A.. Voltage-gated ion channels and gating modifier toxins. Toxicon 49, 124–141, doi: 10.1016/j.toxicon.2006.09.022 (2007).17239913 [OpenAIRE] [PubMed] [DOI]

Davies T. G. E., Field L. M., Usherwood P. N. R. & Williamson M. S. DDT, pyrethrins, pyrethroids and insect sodium channels. IUBMB Life 59, 151–162, doi: 10.1080/15216540701352042 (2007).17487686 [OpenAIRE] [PubMed] [DOI]

Loughney K., Kreber R. & Ganetzky B. Molecular analysis of the para locus, a sodium channel gene in Drosophila. Cell 58, 1143–1154 (1989).2550145 [OpenAIRE] [PubMed]

Zhou W., Chung I. B., Liu Z. Q., Goldin A. L. & Dong K. A voltage-gated calcium-selective channel encoded by a sodium channel-like gene. Neuron 42, 101–112, doi: 10.1016/s0896-6273(04)00148-5 (2004).15066268 [OpenAIRE] [PubMed] [DOI]

Ramaswami M. & Tanouye M. A. Two sodium-channel genes in Drosophila: implications for channel diversity. Proceedings of the National Academy of Sciences of the United States of America 86, 2079–2082, doi: 10.1073/pnas.86.6.2079 (1989).2538830 [OpenAIRE] [PubMed] [DOI]

Thackeray J. R. & Ganetzky B. Conserved alternative splicing patterns and splicing signals in the Drosophila sodium channel gene para. Genetics 141, 203–214 (1995).8536968 [OpenAIRE] [PubMed]

Hanrahan C. J., Palladino M. J., Ganetzky B. & Reenan R. A. RNA editing of the Drosophila para Na(+) channel transcript. Evolutionary conservation and developmental regulation. Genetics 155, 1149–1160 (2000).10880477 [OpenAIRE] [PubMed]

Dong K.Insect sodium channels and insecticide resistance. Invertebrat e neuroscience: IN 7, 17–30, doi: 10.1007/s10158-006-0036-9 (2007).17206406 [OpenAIRE] [PubMed] [DOI]

70 references, page 1 of 5
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