publication . Other literature type . Article . 1971

Albumin standards and the measurement of serum albumin with bromcresol green.

Basil T. Doumas; W. Ard Watson; Homer G. Biggs;
  • Published: 01 Jan 1971
  • Publisher: Elsevier BV
Abstract
Abstract A rapid and reliable method for measuring serum albumin employing bromcresol green is described. The addition of albumin to a solution of bromcresol green in a 0.075 M succinate buffer pH 4.20 results in an increase in absorbance at 628 nm. The absorbance-concentration relationship is linear for samples containing up to 6 g/dl albumin. Bilirubin, moderate lipemia, and salicylate do not interfere with the analysis. The use of a nonionic surfactant (Brij-35) reduces the absorbance of the blank, prevents turbidity and provides linearity. The results by this method agree very well with those obtained by electrophoresis and salt fractionation. The method is ...
Subjects
free text keywords: Clinical Biochemistry, Biochemistry, Biochemistry, medical, General Medicine, Chromatography, Blood chemistry, Chemistry, Bromcresol Green, Blood serum, Bovine serum albumin, biology.protein, biology, Absorbance, Serum albumin, Blood proteins, Albumin
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