publication . Article . 2009

Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants.

Frank Sainsbury;
Open Access English
  • Published: 01 Oct 2009 Journal: VIROLOGY (issn: 0042-6822, Copyright policy)
  • Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract
AbstractTo elucidate the mechanism of formation of cowpea mosaic virus (CPMV) particles, RNA-2-encoded precursor proteins were expressed in Spodoptera frugiperda cells. Processing of the 105K and 95K polyproteins in trans to give the mature Large (L) and Small (S) coat proteins required both the 32K proteinase cofactor and the 24K proteinase itself, while processing of VP60, consisting of the fused L–S protein, required only the 24K proteinase. Release of the L and S proteins resulted in the formation of virus-like particles (VLPs), showing that VP60 can act as a precursor of virus capsids. Processing of VP60 expressed in plants also led to efficient production ...
Subjects
Medical Subject Headings: virusesbiochemical phenomena, metabolism, and nutrition
free text keywords: Virology, Cowpea mosaic virus, Virus-like particle, Empty particle, Coat protein precursor, Proteolytic processing, Insect cell expression, Transient expression
Funded by
EC| PLAPROVA
Project
PLAPROVA
Plant Production of Vaccines
  • Funder: European Commission (EC)
  • Project Code: 227056
  • Funding stream: FP7 | SP1 | KBBE
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