publication . Other literature type . Article . 2010

Cellulase activities in biomass conversion: measurement methods and comparison

Mehdi Dashtban; Miranda Maki; Kam Tin Leung; Canquan Mao; Wensheng Qin;
  • Published: 24 Sep 2010
  • Publisher: Informa UK Limited
Cellulose, the major constituent of all plant materials and the most abundant organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected by β-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases), and β-glucosidases acting in a synergistic manner. In biopolymer hydrolysis studies, enzyme assay is an indispensable part. The most commonly used assays for the individual enzymes as well as total cellulase activity measurements, including their advantages and limitations, are summarized in this review article. In addition, some novel appro...
free text keywords: Biotechnology, Applied Microbiology and Biotechnology, General Medicine
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